Research Technician, NEIDL ARC Service Center. National Emerging Infectious Disease Lab

Boston University•Boston, MA

4d•Onsite

About The Position

The Arboviral Research Core (ARC) is a specialized research core within the National Emerging Infectious Diseases Laboratories (NEIDL) at Boston University. The NEIDL ARC is seeking a highly motivated Research Specialist. The ARC supports the production, maintenance, and infection of arthropod vectors to study the mechanisms of pathogen transmission between vectors and hosts. Operating under Arthropod Containment Level 3 (ACL-3) the ARC conducts complex research that is critical to advancing the understanding of emerging infectious diseases. The successful candidate will participate in multiple projects involving both internal and external collaborators.

Requirements

4-6 years' experience and an associate or bachelor's degree
Virology
Virus propagation and amplification
Virus concentration by ultracentrifugation
Viral quantification using plaque assays, TCID50 assays, and focus-forming assays
Quantitative PCR (qPCR)
Flow cytometry
ELISA
Molecular Biology
Molecular cloning
RNA interference techniques (siRNA and dsRNA)
CRISPR-Cas9 gene editing
Insectary Operations
Mosquito rearing and colony maintenance
Mosquito injection and dissection
Artificial mosquito feeding techniques

Nice To Haves

The ideal candidate must be willing to work with RG-3 pathogens.

Responsibilities

Independently conducting experiments using insect and mammalian cell culture systems, several mosquito species, and mouse models to investigate the transmission and acquisition of arboviruses.
Maintaining accurate laboratory records, analyzing data, and contributing to internal reports, contracts, and project documentation.
Maintaining laboratory and insectary operations in compliance with institutional operational, and biosafety requirements; ordering reagents and supplies.