Research Technician II

Mass General Brigham•Boston, MA

1d•Onsite

About The Position

Carries out a broad range of technically advanced research activities and procedures; evaluates conclusions and has considerable latitude to modify or devise methods and techniques as necessary to achieve desired results; typically supervises technical and sometimes administrative elements of the research unit. The Research Technician II reports directly to the Director, Principal Investigator, and/or managers of the laboratories and is expected to work independently on assigned research with minimal guidance from a higher-level staff member or Principal Investigator. The individual is expected to carry out research activities of moderate complexity requiring some professional training. This involves interacting with patients, clinicians, nurses, OR staff, pathology, radiology and laboratory research personnel. The individual will work with clinicians and director to identify and educate patients to gain consent for sample and data collection for the gyn focused tissue repository. This individual will also be processing samples in the laboratory as per study protocol and assisting with the day-to-day operations of our gyn-fertility focused sample repository. The position is also responsible for standard molecular and biochemical analyses, establishing and maintain cell, organoid and animal experiments, as well as a number of other wet lab-based research activities including supply ordering and sample organization and inventory.

Requirements

Bachelor's Degree Related Field of Study required
Previous Research Lab Experience 1-2 years required
Analytical skills and ability to resolve technical problems.
Ability to interpret acceptability of data results.
Strong Computer skills.
Demonstrated competence in research techniques and methodologies.
Proficient in or acquire the necessary skills to assist clinical and or post doctoral fellows in the initiation and or completion of their various research projects.
Ability to interact professionally with patients both in the clinic and in the pre-op areas on their day of surgery.
Ability to establish and maintain positive relationships with clinicians, nurses, OR staff, radiology and or pathology personnel.
PC and Mac competency is required
Must have the ability to work semi-independently and display initiative and adapt to a flexible schedule.
Excellent communication/interpersonal skills required
Must be resourceful, self motivated, highly organized, have good time management skills, work cooperatively with others and be willing to learn new techniques.

Nice To Haves

Prior experience with mouse handling and mouse based experiments is preferred.
The wet lab techniques required can include but are not limited to: preparation and maintenance of cell and organoid cultures, development of/or utilization of in situ and in vivo models, collection of fresh tissue, protein, lipid and nucleotide isolation and analyses (Northern, Western, EMSA, TLC, kinase assays etc.), flow cytometric analysis, molecular cloning, mutation analyses, site directed mutagenesis, CRISPR, RT-PCR, ELISA, genotyping, immunohistochemistry or immunocytochemistry, in situ hybridization and navigation of basic NCBI sponsored gene and protein data bases and interpretation programs.

Responsibilities

Executes protocols of non-routine experiments.
Assists PI with determining the most suitable methodology.
Performs basic design and modification of protocols.
Calculates, transcribes, and analyzes data.
Prepares and presents reports.
Organizes and summarizes acquired data using scientific and Statistical Techniques.
Participates in the design of experiments or field work.
Interact with patients undergoing a fertility or gynecologic related procedure at MassGeneral Brigham.
Communicate with them about our biorepository and educate them on our research objectives.
Obtain informed consent from these patients for tissue, blood, and/or other body fluid collection, along with clinical information.
Coordinate blood (or other body fluids urine, ascites, etc.) and or tissue collection with clinicians, nurses, OR staff, radiology and or pathology personnel.
Establish and maintain organoid lines derived from primary human tissue.
Establish primary derived xenografts from fresh or cryopreserved malignant tissue.
Navigate and keep up to date our Women’s Health Directory and RedCap databases that store patient information, sample location, and distribution logs.
Help prepare, submit and maintain IRB approval for our banking and research protocols.
Submit continuing reviews and amendments for any research updates.
Research and collect basic study data related to assigned projects as directed through laboratory experiments and or medical record investigation as approved by the institutional review board.
Facilitate the experiments of Clinical Research Fellows, Postdoctoral Research Fellows, Technicians and other research personnel.
Assist with gathering information needed for grant preparation and reporting.
Meet weekly with the Principal Investigator to discuss results, plan and implement experiments, and discuss new areas of interest.
Attend all applicable laboratory and general VCRB meetings.
Maintain accurate, legible research e-notebooks.
Analyze and post results in notebooks within 24 hours of completing the experiments.
Perform statistical analysis of results; prepare graphs, figures, and posters for oral and written presentations.
Instruct students, fellows, and other research personnel in routine laboratory techniques.
Operate and maintain various moderately complex lab equipment related to research activities.
Participate in VCRB, departmental and laboratory training, and educational events (e.g., chemical and biosafety training, animal care practices, etc.).
Practice laboratory safety following MGB and VCRB guidelines.
Interact constructively with all laboratory personnel of the Vincent Center for Reproductive Biology, the MGB Department of Obstetrics and Gynecology, and MGB Cancer Center.
Maintain a professional demeanor within the laboratory and when attending scientific functions or meetings.