RESEARCH TECHNICIAN II

Duke Careers•Durham, NC

1d•Onsite

About The Position

The Patel Lab, within the Department of Neurosurgery, Preston Robert Tisch Brain Tumor Center and the Center for Advanced Genomic Technologies, focuses on single-cell genomics in brain tumors. The lab's research includes single-cell analyses of patient samples, development of rationally designed cellular therapies, creation and study of patient-specific tumor models to understand minimal residual disease and tumor evolution during therapy, and the use of functional genomics to understand tumor dynamics. The lab is seeking a technician to perform a variety of molecular biology-based assays to support multiple projects. This position will specifically support work in the development of cellular therapies for glioblastoma. Activities will include complex tissue culture, maintenance and transduction of T-cell cultures, tissue processing, in vivo experiments using mouse models of glioblastoma, single-cell RNA-sequencing and library generation, cloning, and other molecular biology techniques.

Requirements

Tissue culture including cell lines and primary human T cells
Mouse survival surgeries and mouse colony management
Molecular biology techniques such as cloning and flow cytometry
Next generation sequencing library preparation
Bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.
No experience required above education/training requirement, or an equivalent combination of relevant education and/or experience.

Responsibilities

Lab maintenance (ordering, making common reagents/buffers, competent cells, basic tissue culture maintenance)
Experimental work related to lab activities including tissue culture, cloning, sample preparation for next generation sequencing under supervision from graduate students/postdoctoral fellows
Oversight over mouse colony and animal activities.