Research Technician II

Children's Hospital of Philadelphia•Philadelphia, PA

8d•$43,900 - $54,800

About The Position

This entry-level research technical role participates in planned research projects by performing research protocols, experimental procedures, and other laboratory tasks under direct supervision. The technician may occasionally use minimal discretion and judgment in executing and interpreting experiments and their results. The position is in the laboratory of Dr. Judith Kelsen and Dr. Alyssa Baccarella in the Division of Gastroenterology, Hepatology and Nutrition at Children’s Hospital of Philadelphia, with a focus on Very Early Onset Inflammatory Bowel Disease (VEO-IBD). Work includes preparation for single-cell RNA sequencing, isolation of peripheral blood mononuclear cells (PBMCs), flow cytometry, and other laboratory activities. The technician maintains laboratory notebooks, records, and protocols and is expected to be self-driven, organized, communicative, and committed to learning and teamwork.

Requirements

High school diploma or GED.
At least one year of related classroom experience or successful completion of a technical training program (such as Wistar or WPSI) and research laboratory experience.
Intermediate understanding of laboratory procedures, protocols, and equipment.
Basic exposure to laboratory-specific experiments, techniques, and scientific analysis.
Excellent verbal and written communication skills.
Excellent organizational skills.
Solid critical-thinking and problem-solving skills.
Solid analytical skills.
Effective data-recording skills and the ability to work with a team.

Nice To Haves

Associate's degree.
Bachelor's degree.
At least two years of related classroom experience or successful completion of a technical training program (such as Wistar or WPSI) and research laboratory experience.
Prior experience with single-cell RNA sequencing preparation, PBMC isolation, or flow cytometry.

Responsibilities

Perform routine tests and experiments under the direction of a principal investigator, supervisor, or designee.
Perform research procedures on experimental samples as directed.
Record and check experiments and results for completeness and accuracy.
Perform calculations and maintain appropriate logs and laboratory notebooks.
Perform tasks requiring familiarity with fundamental techniques and laboratory equipment.
May assist with planning specific research protocols and procedures.
Maintain office and laboratory areas, solutions, reagents, supplies, and equipment; dispose of specimens, media, and other materials using appropriate procedures.
Assist with care and maintenance of laboratory equipment.
May coordinate schedules of tests and procedures.
Attend courses, seminars, and laboratory meetings to expand knowledge of research and laboratory techniques.
Record results, organize data, and maintain laboratory notebooks in an organized manner.
Compile and help analyze data and prepare charts, graphs, and study results as directed.
Conduct literature searches as directed.
Prepare for single-cell RNA sequencing, isolate PBMCs, and perform flow cytometry as part of research on VEO-IBD.
Maintain appropriate laboratory notebooks, records, and protocols.