Research Technician II

University Health NetworkToronto, ON
CA$31 - CA$39Onsite

About The Position

The Princess Margaret Genomics Centre is seeking a Temporary Full-time Research Technician II. Working under the direction of senior staff, the successful applicant will utilize standardized laboratory techniques and procedures in genomics and molecular biology applications; perform experiments and analysis; communicate and maintain records of experimental data. As an integral team member at the Genomics Centre, a UHN core facility, the research technician must be dedicated and result-driven and will be involved in all aspects of service projects, following SOPs and meeting target deadlines. There will be a special emphasis on techniques for single cell and single nucleus transcriptional profiling assays, especially 10x Genomics Flex.

Requirements

  • At minimum, a Bachelors degree in genomics, molecular biology or related discipline is required
  • At least 3 years of experience with standard molecular biology laboratory techniques is required, such as: PCR reaction set up and purification.
  • Nucleic acid quantification by spectrophotometry and fluorimetry
  • Handling of single channel and multichannel pipettes.
  • Manual counting of cells and nuclei via cytometer.
  • A willingness to learn new techniques and technologies is required.
  • Demonstrated excellent organizational and time management skills are required.
  • Ability to work independently and accurately under deadlines is required.
  • Meticulous and detail-oriented lab performance is required.
  • Excellent interpersonal and communication skills in a team environment is required.

Nice To Haves

  • Experience with 10x Genomics single cell profiling assays is an asset.
  • Experience with nuclei extraction from fresh or frozen solid tissue and cells is an asset.
  • Experience with using LIMS (Laboratory Information Management System) is an asset.
  • Experience with Illumina DNA sequencing library construction workflows is an asset.

Responsibilities

  • Will utilize standardized laboratory techniques and procedures in genomics and molecular biology applications
  • Will perform experiments and analysis; communicate and maintain records of experimental data
  • Will be a key member of a team focused on single cell and single nucleus genomics

Benefits

  • Competitive offer packages
  • Government organization and a member of the Healthcare of Ontario Pension Plan (HOOPP)
  • Close access to Transit and UHN shuttle service
  • A flexible work environment
  • Opportunities for development and promotions within a large organization
  • Additional perks (multiple corporate discounts including: travel, restaurants, parking, phone plans, auto insurance discounts, on-site gyms, etc.)
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