Research Specialist

University of Chicago•Austin, TX

2d•Onsite

About The Position

The Simic group at the Pritzker School of Molecular Engineering is seeking a Research Specialist interested in gaining experience in synthetic biology and cell engineering. Under the direction of Assistant Professor Milos Simic, the Specialist will help get the lab started and apply cutting-edge approaches to develop next-generation cell therapies, including smart immune cells designed to recognize and kill cancer cells or to treat other challenging diseases such as neuroinflammation and neurodegeneration. The selected candidate will contribute to a team in the performance of laboratory and investigative procedures by conducting a variety of experimental techniques such as molecular cloning, cell culture (cell lines and primary cells), flow cytometry, and ELISA-like assays. The successful candidate will receive detailed technical training and will execute cellular assays to collect, analyze and compile data in support of our research. Additionally, the Specialist will be expected to assist with managing day-to-day lab operations, perform general lab duties, and be a positive lab citizen. This is an outstanding opportunity for a highly motivated candidate seeking in-depth experience in cutting-edge biomedical research.

Requirements

Minimum requirements include a college or university degree in related field.
Minimum requirements include knowledge and skills developed through < 2 years of work experience in a related job discipline.
Knowledge of basic computer proficiency including computer programs and standard laboratory equipment.
Knowledge of relevant scientific field.
Knowledge of regulatory policies and procedures.

Nice To Haves

Bachelor’s degree in a related field, such as biology, immunology, chemistry, etc.
Design and assembly of DNA constructs, including modern molecular cloning techniques like Golden Gate or Gibson Assembly.
Familiarity with viral vectors, transposon vectors and/or CRISPR genome integration techniques for genetic engineering.
Familiarity with CRISPR-Cas9 screening approaches, including genome-wide or targeted pooled screens, for functional genomics studies.
Culture mammalian cells, including tumor cell lines, primary immune cells , including T cells, and/or stem cells.
Background with animal experiments, including mouse models for cancer, autoimmunity, and/or neurodegeneration (subcutaneous and intravenous injection).
Understanding of immunology assays, such as cytotoxicity, cytokine secretion and general in vitro functional measurements of T cells.
Understanding of fluorescence microscopy and imaging data analysis.
Comprehension of spatial transcriptomics technologies, such as Visium, MERFISH, Slide-seq, and associated computational analysis pipelines.
Proficient in flow cytometry and cell sorting.
Knowledge of basic programming in a few coding languages.
Familiarity with first principles simulation techniques.
Problem-solving, organization, analytical and coordination skills including attention to detail.
Excellent oral and written communication skills.
Read and comprehend scientific and technical literature and methods, to stay abreast of developments in the field.
Work both independently and in collaboration with other lab personnel

Responsibilities

Performs various investigative procedures and operates a variety of instruments and other scientific equipment. Examples of equipment routinely used are plate readers for absorbance or luminescence-based assays, microscopes for live cell functional studies, PCR machines for gene amplification and cloning, and flow cytometers and cell sorters for single-cell analysis and isolation.
Collaborates with internal and external students, scientists, researchers, programmers, and engineering teams.
Contributes new ideas that can lead to new experiments and research grant proposals.
Helps develop, design, and conduct research projects in accordance to plans.
With moderate supervision, manages research-related duties and tasks.
Documents research efforts and software adequately for sharing with the PI and lab members.
Helps disseminate research results in high-impact journals and conferences.
Conducts periodic training for current or new members of the research group.
Recognizes abnormal or potentially abnormal results and communicates about them and sets up procedures to investigate and correct problems.
Ensures project compliance with different policies, procedures, directives, and mandates.
Complies with institution, state and federal regulatory policies, procedures, directives, and mandates.
Analyzes and maintains data and/or specimens.
Conducts literature reviews.
Assists with preparation of reports, manuscripts and other documents.
Maintains recruiting and scheduling research subjects; assisting with developing or amending study protocols; assisting with developing data collection tools; assisting with building databases; and providing general administrative support.
Has general awareness in research techniques or methods, regulatory policies and procedures, and relevant scientific field.
Performs other related work as needed.