UNIV - Research Specialist III - Pharmacology & Immunology

Medical University of South Carolina•Charleston, SC

17h•Onsite

About The Position

The Paczesny Laboratory in the Hollings Cancer Center and the Department of Pharmacology and Immunology at the Medical University of South Carolina has an open position for a Research Specialist III. Our laboratory integrates in vivo murine models and human clinical samples to elucidate mechanisms of immunity and tolerance in myeloid malignancies and hematopoietic cell transplantation (HCT). A central aspect of this position is hands-on work with both animal models and human biospecimens, and candidates must be comfortable and experienced in working in both settings.

Requirements

A bachelor's degree in chemistry, biology, or other natural, life or health care science directly related to the assigned area of research and two years advanced journel-level work experience in a clinical or research setting.
Must have previous experience in a laboratory.

Nice To Haves

Ideally a master’s in biology or immunology with at least 3 years of experience in this field.
Strong organizational, record keeping, interpersonal and communication skills required.
Must be able to perform complex calculations, to follow complicated experimental procedures and methods and develop independence with tasks and experiments.
Prior experience working with patients’ samples handling as well as mice handling, and flow cytometry is valued.
Some weekend, holidays and/or evenings may be required.
Must be organized and have attention to details.
All personnel are expected to maintain laboratory notebooks and store all the data in the lab shared drive.
Ability to work rotating shifts as required.
Ability to work overtime as required.
Ability to learn and use new processes, tools and equipment as required.

Responsibilities

Perform routine handling of mice including monitoring, treatments, and experimental procedures.
Assist with breeding, colony management, and genotyping of transgenic.
Maintain accurate animal records and ensure compliance with institutional animal care and biosafety protocols.
Assist with intravenous and intraperitoneal injections of novel therapeutics (cellular of antibody-based).
Perform immunologic experiments on murine models (e.g., harvest organ, digest, flow cytometry, confocal, etc).
Immunology experimentation employing various techniques, including isolation and purification of T cells, and regulatory T cells.
Sort T cells and other hematopoietic or immunes cells.
Perform isolation of cells, cell culture and in vitro functional assays.
Harvest murine organs, including spleen, liver, bone marrow, and lungs.
Perform tissue digestion and preparation of single-cell suspensions.
Maintain cell lines; viability, cytotoxicity assay; transfection and infection, etc.
Prepare patients samples per lab good practice and perform immunologic experiments (e.g., high-throuput ELISA, flow cytometry, etc).
These responsibilities include receiving or shipping samples, organizing them, and storing them in freezer following lab established freezer maps.
Thaw precious plasma or PBMCs patients’ samples following rigorous protocols and perform sequential ELISA or flow cytometry and analysis.
Assist with daily lab operations including routine maintenance of and experimentation in mice.
The selected candidate will assist with daily lab operations including stocking consumable items and reagents, making buffers, and aliquoting reagents.
Fill liquid nitrogen to keep frozen cells alive.
Autoclave the biologic trash and prepare and maintain lab work (e.g., fill and autoclave tips, clean hood and incubator).
Facilitate general maintenance of the research space by working with MUSC to ensure that the laboratory area is maintained and that repairs, waste disposal, and cleaning needs are addressed in a timely manner.
Collect research experimental data and analyze results and generate figures.
Additional duties assigned by the Principal Investigator.
This will include helping refine or prepare lab protocols, maintain IBC and IACUC protocols up to date.
Train new lab members for basic experiments.