UNIV - Research Specialist II - Pharmacology & Immunology

Medical University of South Carolina•Charleston, SC

2d•$45,300 - $77,000•Onsite

About The Position

The Research Specialist II will work closely with the Principal Investigator to generate high-quality data for high impact publications, conference presentations and clinical translation. The ideal candidate will have prior hands-on experience with cell culture, DNA/RNA processing, molecular cloning, gene transduction, flow cytometry, and handling mice. Duties include cell culture, molecular cloning, PCR, T cell transduction, flow cytometry, in vitro functional assays of immune effector cells, and measuring antitumor activity in mice.

Requirements

A bachelor's degree in chemistry, biology, or other natural, life or health care science.
One year work experience directly related to the assigned research area.

Nice To Haves

Master's degree in basic science or a related field.
One year of relevant experience.

Responsibilities

Execute experiments involving T cell isolation, activation, viral transduction and expansion.
Perform CAR T cell functional assays such as repeat killing, cytokine production, CAR phenotyping, and immunofluorescent staining.
Detect CAR expression, perform lentivirus and retrovirus production.
Isolate RNA and DNA from samples and process samples for single cell RNA sequencing.
Perform molecular cloning, miniprep and maxiprep of DNA.
Design and run PCRs.
Measure cytokine production using ELISA or Luminex assays.
Perform and analyze experiments including multi-parameter flow cytometry and IncuCyte.
Organize lab space and keep track of lab reagents and materials.
Perform cell therapy experiments in mice.
Measure BLI.
Process peripheral blood, and isolate mononuclear cells and specific cell subsets.
Keep mouse records and mouse inventory.
Perform mouse IV, SC and IP injections.
Monitor mice throughout experiments.
Collect and process tumors.
Perform mouse surgeries as needed.
Maintain lab records of reagents and orders.
Generate reports and summarize experimental results.
Maintain lab notebook updated.
Keep record of lab SOPs, receive lab orders.
Assist in training junior staff on experimental techniques.