Research Specialist A/B

University of Pennsylvania Perelman School of Medicine•Philadelphia, PA

2d•$45,278 - $67,409•Onsite

About The Position

The Raabe Laboratory at the Perelman School of Medicine, University of Pennsylvania, is seeking a highly motivated Research Specialist A/B to join our research program focused on liver disease, fibrosis, and regenerative medicine. The work includes a variety of cutting-edge molecular and cell biologic techniques to investigate the role of senescence and other fibrosis related pathways in human liver derived organoids and primary human cells in the context of liver disease. Relevant diseases include viral infections and age related fibrotic liver diseases. This work will involve genome editing using CRISPR/Cas9 and siRNA techniques both in organoids /cells and in related mouse models - mostly performed by using lipid nanoparticles. The work involves basic cell culture experimentation (including cell transfection/transduction, stimulation and time course collection), molecular biologic assays (SDS-PAGE, Immunoblotting), and fluorescence microcopy as well as basic data quantification and analysis. The position will also involve assisting with maintaining mouse models, lab organization through ordering of equipment/reagents, maintaining stocks, reagents, chemicals, records, and lab instruments, and attending planning and review meetings. Other duties as assigned. The Research Specialist B position will undertake the duties outlined for the Research Specialist A position, with the additional expectation that the candidate will work independently to develop novel methods and techniques, in addition to undertaking established methods and techniques. This position would also have the added expectation of performing some methods/techniques/data analysis independently, and have a background including more experience, specifically with skills necessary to do data analysis and compilation using appropriate software.

Requirements

Bachelor of Science and 0 to 1 year of experience or equivalent combination of education and experience is required (for Research Specialist A).
A Bachelor's Degree is required and 1 to 2 years of experience or equivalent combination of education and experience (for Research Specialist B).
Prior experience with mammalian cell culture, molecular/cell biology is strongly preferred (for Research Specialist A).
Prior experience with mammalian cell culture and molecular/cell biology is required (for Research Specialist B).
Prior experience with mouse related work preferred (for Research Specialist B).
Ability to work independently and apply critical thinking and sound judgment.
Excellent organizational and time management skills.
Ability to work with a multi-disciplinary team.

Nice To Haves

Genome editing using CRISPR/Cas9 and siRNA techniques.
Experience with lipid nanoparticles.
Basic cell culture experimentation (including cell transfection/transduction, stimulation and time course collection).
Molecular biologic assays (SDS-PAGE, Immunoblotting).
Fluorescence microscopy.
Basic data quantification and analysis.
Skills necessary to do data analysis and compilation using appropriate software (for Research Specialist B).

Responsibilities

Perform a variety of genetic, molecular, and cell biologic techniques to characterize the role of senescence in human liver derived organoids including CRISPR and siRNA - based genome editing.
Assist with related mouse experiments.
Help with lab organization by ordering reagents and materials, maintaining lab stocks, keeping records, and maintaining lab instruments.
Develop novel methods and techniques (for Research Specialist B).
Perform methods/techniques/data analysis independently (for Research Specialist B).
Maintain mouse models.
Order equipment/reagents.
Maintain stocks, reagents, chemicals, records, and lab instruments.
Attend planning and review meetings.
Other duties as assigned.

Benefits

Comprehensive medical, prescription, behavioral health, dental, vision, and life insurance benefits.
Flexible spending accounts for health care and dependent care expenses.
Tuition assistance for employees, spouses, and dependent children at Penn and other institutions.
Generous retirement plans (Basic, Matching, and Supplemental) with pre-tax or Roth options.
Substantial amount of time away from work (vacation, personal affairs, illness, family time).
Long-Term Care Insurance.
Wellness and Work-life Resources.
Professional and Personal Development resources.
Access to a wide range of University resources, cultural and recreational activities.
Discounts on arts and entertainment, transportation, mortgages, goods, and services.
Flexible work options (non-traditional work hours, locations, and/or job structures).
Forgivable loan for eligible employees interested in buying a home or residing in West Philadelphia.
Adoption Assistance reimbursement for qualified expenses.