Research Senior Technician
About The Position
The Research Senior Technician will make common buffers, maintain insect and human cell lines, clone genes into plasmids, purifies plasmids and bacmids, over-expresses recombinant proteins in E. coli, insect cells and human cell lines, purifies recombinant proteins using FPLC system, transcribes RNAs in vitro, extracts RNAs, performs qRT-PCR, quantifies mRNA levels by Dual-Luciferase Reporter assay, and prepares samples for structural biology. The Research Senior Technician will record the experimental work on the lab notebook; order the reagents and items through Workday; organize the lab supplies; cleans the FPLC; cell homogenizer and other lab equipment; updates the reagent excel spreadsheets; read literature and papers relevant to the research; other duties as assigned.
Requirements
- Bachelor’s degree in Biochemistry or molecular biology in a biological/health sciences related field or equivalent experience
- No prior experience required with a Bachelor’s degree
- Two (2) years of relevant experience with an Associate’s degree
- Four (4) years of relevant experience with a High School diploma
- Clear oral and written communication skills in English
- Desire to work as part of a highly motivated team
Nice To Haves
- Expertise in cell biology, especially gene transfections in human cell line and immunoprecipitation of the proteins
- Experience in protein purification
- 3-4 years of relevant experience preferred
Responsibilities
- Make common buffers
- Maintain insect and human cell lines
- Clone genes into plasmids
- Purify plasmids and bacmids
- Over-express recombinant proteins in E. coli, insect cells and human cell lines
- Purify recombinant proteins using FPLC system
- Transcribe RNAs in vitro
- Extract RNAs
- Perform qRT-PCR
- Quantify mRNA levels by Dual-Luciferase Reporter assay
- Prepare samples for structural biology
- Record experimental work on the lab notebook
- Order reagents and items through Workday
- Organize lab supplies
- Clean the FPLC, cell homogenizer and other lab equipment
- Update reagent excel spreadsheets
- Read literature and papers relevant to the research
- Other duties as assigned
Benefits
- Medical, dental and vision coverage, with Ohio State paying a significant portion of the cost.
- Paid time off, including sick and vacation time and 11 holidays.
- State retirement plan or an alternative retirement plan, both with generous employer contributions.
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What This Job Offers
Job Type
Full-time
Career Level
Senior
