Research Lab Tech I, Hematology Hollenhorst Lab

Mass General Brigham•Boston, MA

2d•Hybrid

About The Position

The Hollenhorst Lab applies innovative biochemical strategies to study clinically relevant questions regarding the proteins that regulate bleeding and clotting. The goal is to further our understanding of the basic biology of hemostasis and thrombosis with an eye towards questions directly relevant to the care of patients. We are looking for an enthusiastic and motivated individual to perform experiments under the supervision of the principal investigator or senior lab member. The selected candidate will perform western blots, immunoprecipitation, protein purification, and prepare samples for mass spectrometry analysis. The individual will work with a high degree of independence, organize notebooks and manage data, and perform other laboratory experiments relevant for understanding platelets and coagulation factors. Ideal candidates will have training in wet lab techniques, strong organizational skills, and be motivated to further their career by learning and adopting new skills. While applicants from diverse backgrounds and career stages are welcome, this position is a particularly good fit for individuals who seek a 1-2 year research experience prior to applying to PhD and/or MD programs. The principal investigator will provide direct mentorship and long-term career advice. For individuals with an interest in gaining clinical experience in advance of applying to medical school, the principal investigator will provide shadowing opportunities.

Requirements

Bachelor's degree required. in biology, chemistry, or a related scientific field highly preferred.
Previous experience (6 months+) with techniques in experimental biology, chemistry and/or biochemistry preferred.
A positive attitude and eagerness to learn.
Comfortable with working independently and in a team.
Good oral and written communication skills.
Ability to learn and perform a variety of laboratory protocols, to identify potential problems and troubleshoot solutions.
Strong organizational skills.
Ability to logically present data.
Sound judgment regarding time-management and laboratory safety.

Nice To Haves

Prior training in western blot, immunoprecipitation, protein purification by liquid chromatography, flow cytometry, and preparation of samples for mass spectrometry analysis is helpful but is NOT required.

Responsibilities

Perform routine and non-routine experiments, which are moderate to complex, with increasing independence. These include western blot, immunoprecipitation, protein purification by liquid chromatography, flow cytometry, and preparation of samples for mass spectrometry analysis.
Process platelets and plasma samples from donors and patients.
Maintain long-term tissue cultures of human cell lines, monitor cells for viability and contamination.
Maintain a detailed electronic laboratory notebook to reproduce experiments precisely.
Consult with the principal investigator and implement adjustments in experimental design.
Prepare laboratory reagents and chemicals, organize storage. Meticulously maintain and update databases of laboratory supplies and storage. Order new supplies on time to maintain inventory.
Perform routine maintenance and calibration of laboratory equipment, if necessary.
Become proficient in Excel, FlowJo, and GraphPad Prism as needed analyze and critically evaluate data.
Present data and relevant research publications in lab meetings. Help to prepare presentations and written articles for publication.
All other duties, as assigned.