Research Associate/Senior Research Associate, Cell Engineering

Colossal Biosciences•Dallas, TX

1d•Onsite

About The Position

An affiliate of Colossal is looking for a talented research associate in cell biology with an interest in the derivation, characterization, and gene editing in non-model mammalian organisms. You will work closely with other lab team members in efforts to innovate on protocols of genome editing, reprogramming and lineage differentiation using cutting edge technologies. This position is based on-site in Dallas, TX. Relocation assistance is available.

Requirements

Experience in primary cell line derivation, culture optimization and cryopreservation
Experience with electroporation and transfection to maximize gene delivery and viability
Experience immunostaining cells for flow cytometry and fluorescent microscopy
Exceptional aseptic technique as antibiotic free cultures are standard
Organized and comfortable multi-tasking to deliver on concurrent projects on time
Strong written and oral communication skills, as well as creative problem-solving skills

Nice To Haves

Experience with high throughput imaging platforms and data analysis
Experience with automated cell culture systems
Experience with stem cell culture, such as mouse embryonic stem cells (mESC) or induced pluripotent stem cells (iPSC)

Responsibilities

Derive and characterize stable cell lines from primary tissues
Microdissection skills for cell line derivations
Test novel cell culture conditions and delivery methods to optimize gene editing
Expand clonal cell lines for cryopreservation, genotyping and QC assays
Work with team members to interrogate targeting, genotyping and QC workflows
Assist with making edited cell lines that meet quality control criteria for downstream SCNT
Schedule, analyze and execute experiments to deliver on project milestones
Attend lab meetings, prepares reports and presentations on the progress of projects
Demonstrate great organizational skills and excellent laboratory record maintenance