Research Assistant - Microbiology and Immunology (Weber Lab)

University of Iowa • Iowa City, IA

13h • Onsite

About The Position

This Research Assistant position is available in the laboratory of Dr. Mary Weber in the Department of Microbiology and Immunology. The successful candidate will contribute to cutting-edge research focused on understanding how the intracellular bacterial pathogens Chlamydia trachomatis and Orientia tsutsugamushi use secreted effector proteins to manipulate host cell processes and promote infection. Research projects will utilize a multidisciplinary approach incorporating molecular biology, cell biology, biochemistry, microbiology, and genetic techniques.

Requirements

• Requires a bachelor’s degree in Microbiology, Molecular Biology, Cell Biology, or Immunology, or a related field or equivalent experience
• Excellent written and verbal communication skills
• Basic knowledge of each of the following: General microbiologic techniques, such as culture, storage, and safe handling of pathogenic agents.
• Basic knowledge of each of the following: Standard molecular techniques, including PCR, plasmid purification, bacterial transformation, agarose gel electrophoresis, and western blotting.
• Basic knowledge of each of the following: Gathering, recording, and analyzing of experimental data.
• Basic knowledge of each of the following: Data analysis and presentation software, such as Microsoft Excel, Prism, Microsoft Word, and Photoshop
• Proficiency with each of the following: Ability to effectively prioritize projects to consistently meet deadlines
• Proficiency with each of the following: Ability to multitask in a fast-paced environment
• Proficiency with each of the following: Attention to detail and accuracy
• Proficiency with each of the following: Ability to work in a team

Nice To Haves

• Prior experience working with pathogenic microorganisms is desired
• Prior experience with each of the following: Tissue culture experience
• Prior experience with each of the following: Immunofluorescence microscopy
• Prior experience with each of the following: Western Blotting
• Prior experience with each of the following: Cloning

Responsibilities

• Perform library and online research on specified topics for use in experimentation.
• Perform experimental studies based on given hypotheses and goals; evaluate and record outcomes of experiments based on hypotheses and goals and submit for investigator’s inspection.
• Assist in the analysis and interpretation of experiments.
• Observe techniques in other laboratories for implementation in our laboratory.
• Complete work within specified time guidelines; discuss progress and any problems as needed.
• Maintain and passage multiple cell lines.
• Purify chlamydia from infected cells by density gradient.
• Use immunofluorescent microscopy to titer bacterial stocks.
• Perform molecular biology experiments including cloning, construction of vectors for expression in chlamydial and make mutants using site-specific gene inactivation.
• Prepare chemical solutions and media according to established protocols.
• Maintain bacterial and eukaryotic stocks and inventory.
• Present research progress at laboratory and journal club meetings.
• Assist in the preparation of manuscripts and grants by organizing data and preparing images, figures, and tables as directed.
• Assist in completion of required paperwork for new and departing laboratory personnel, including annual training, biohazard safety, laboratory safety, and fire safety training.
• Assist in maintaining accurate records of laboratory databases (equipment and supplies, reagents, microbial strains and stocks, and animals).
• Maintain laboratory supplies and equipment, including the timely ordering, receiving, and proper storage of supplies and equipment.
• Assist in maintaining general laboratory functionality.
• Assist in developing new protocols.
• Maintain a clean work area.
• May assist in the BSL3.
• May assist in animal facilities.

Benefits

• Regular salaried position located in Iowa City, Iowa
• Fringe benefit package including paid vacation; sick leave; health, dental, life and disability insurance options; and generous employer contributions into retirement plans

What This Job Offers

• Job Type: Full-time
• Career Level: Entry Level