Research Assistant IV - Pediatric Surgery - Bondoc Lab
About The Position
The Bondoc Laboratory is seeking a highly motivated and experienced Research Assistant to support research focused on pediatric liver cancer, liver development, stem cell biology, and organoid models. The successful candidate will independently perform complex laboratory experiments, support animal studies, analyze data, and contribute to collaborative research projects. The role includes designing, performing, troubleshooting, and optimizing experiments; executing and adapting protocols; analyzing and interpreting results; maintaining laboratory records; and contributing to publications, presentations, and other research activities. Laboratory work includes cell culture, molecular biology assays, histology, microscopy, and flow cytometry-related sample preparation. The Research Assistant will also support mouse colony care and in vivo studies, maintain laboratory equipment and supplies, ensure compliance with institutional and safety requirements, and help with training and mentoring students, trainees, and junior staff.
Requirements
- Bachelor’s degree in a related field and 3 years of experience in a related job discipline, or a master’s degree in a related field and 2 years of experience.
- Experience with mammalian cell culture, primary cell culture, and human iPSC culture.
- Experience with PCR, qPCR, and Western blotting.
- Experience with histology, immunohistochemistry, immunofluorescence, and microscopy-based analyses.
- Experience preparing and processing samples for flow cytometry and related applications.
- Experience with animal handling, monitoring, tissue collection, and surgical procedures.
Nice To Haves
- Human iPSC culture and differentiation.
- Organoid culture systems.
- Flow cytometry and cell sorting.
- Histology and immunostaining.
- Confocal microscopy.
- qPCR and Western blotting.
- Mouse handling, colony management, and survival surgery.
Responsibilities
- Design, perform, troubleshoot, and optimize laboratory experiments.
- Execute and adapt research protocols to meet project objectives.
- Analyze and interpret experimental data.
- Maintain accurate laboratory records and documentation.
- Contribute to publications, presentations, and other research activities.
- Perform mammalian cell culture, primary cell culture, and human iPSC culture.
- Conduct molecular biology assays, including PCR, qPCR, and Western blotting.
- Perform histology, immunohistochemistry, immunofluorescence, and microscopy-based analyses.
- Prepare and process samples for flow cytometry and related applications.
- Assist with the development, optimization, and implementation of new laboratory methods.
- Maintain research mouse colonies.
- Perform animal handling, monitoring, tissue collection, and surgical procedures.
- Support in vivo studies and associated data collection.
- Maintain laboratory equipment, supplies, and inventory.
- Troubleshoot technical and equipment issues.
- Ensure compliance with institutional, safety, biosafety, and animal care requirements.
- Support laboratory organization and day-to-day operations.
- Train and mentor students, trainees, and junior staff.
- Serve as a technical resource within the laboratory.
- Contribute to a collaborative and productive research environment.
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What This Job Offers
Job Type
Full-time
Career Level
Mid Level
