Research Assistant IV (Molecular Biology and Protein Engineering)

Scripps Research • San Diego, CA

4h • $75,000 - $93,750 • Onsite

About The Position

We are seeking an exceptional Research Assistant candidate to join our molecular biology and protein engineering team working to advance transformative therapies from concept to clinic. In this role, you will collaborate with a dynamic team of protein scientists and molecular biologists to produce proteins, plasmids, and viral vectors that power innovative R&D programs in immuno-oncology, autoimmunity, and gene therapy. Beyond core biologics programs, you will also have the unique opportunity to support diverse initiatives spanning infectious diseases, neglected tropical diseases, and metabolic disorders—reflecting Calibr-Skaggs’ commitment to global health and broad disease indications. Our open lab design fosters seamless collaboration across interdisciplinary teams, while structured growth pathways ensure exceptional talent thrives within the Calibr-Skaggs family. Hands-on wet lab work is central to this position, with opportunities to apply advanced molecular biology and protein engineering techniques to real-world therapeutic challenges.

Requirements

• Bachelor’s degree with 7+ years’ experience, master’s degree with 2+ years’ or PhD with 0+ years’ experience in protein chemistry, chemical biology, biochemistry, or relevant scientific discipline.
• Molecular biology techniques, including construct design, DNA isolation and purification, and assembly-based cloning are required.
• Protein expression in HEK and/or CHO cells is required.
• Protein purification techniques are required, including a basic understanding of FPLC, HPLC, gravity drip columns, and a range of affinity and capture technologies including SEC, IEX, mixed-mode, etc.
• An understanding of biophysical characterization of proteins is important.
• Outstanding organizational skills and experience in preparing scientific and technical documents are important.
• Demonstrated excellence in designing and conducting hands-on experiments, organizing and managing projects, and maintaining meticulous documentation.
• Highly motivated and creative with a devotion to project goals and timelines.
• Proficient in collaborating and communicating effectively with colleagues and management teams.
• Ability to meaningfully contribute to scientific discourse.

Nice To Haves

• Industry experience is preferred, but outstanding candidates without this will be considered.
• Experience with yeast and insect expression platforms is advantageous.
• Medium- and high-throughput protein expression is not required but advantageous.

Responsibilities

• Design bispecific and multi-specific antibodies for immune-oncology and autoimmune diseases.
• Design and clone genes into vectors for mammalian, coli, insect, or yeast expression.
• Protein expression primarily in mammalian cells, sometimes in coli, insect cells, or yeast.
• Protein purification by affinity chromatography, gel-filtration, and other chromatography techniques (ion exchange, hydrophobic interaction chromatography, etc.).
• Protein characterization by SDS-PAGE, analytical SEC-HPLC, LCMS, biolayer interferometry (BLI) and other methods.
• Documentation of plasmids and proteins in the database.
• Presentation of results in group meetings and communication with team members.
• Perform other related duties, tasks and responsibilities as required or assigned.

Benefits

• Eligibility for retirement plans with employer contribution.
• Full suite of Health and Welfare plans including three medical plan options (including an HSA available option), dental, vision, life insurance, disability, EAP and more.
• Access to Flexible Spending Accounts (Medical/Dependent Care).
• Competitive vacation and sick leave policies.
• Free, on-site Counseling Center which provides confidential counseling services to benefits-eligible employees and their family members.
• Free, on-site parking.

What This Job Offers

• Job Type: Full-time
• Career Level: Mid Level