Research Assistant 2

McGill University•Hamilton, ON

2d•Onsite

About The Position

This position is responsible for maintaining and managing Drosophila melanogaster stocks, including setting genetic crosses, collecting progeny, and performing routine husbandry. The role involves preparing and processing Drosophila tissues for downstream analyses, performing immunofluorescence staining and whole-mount in situ hybridization experiments. The Research Assistant will operate laser-scanning confocal microscopy systems, process and analyze confocal images, and perform RNA isolation. Additionally, the position includes carrying out reverse transcription quantitative PCR (RT-qPCR), preparing and analyzing polytene chromosome squashes, maintaining detailed laboratory notebooks and electronic records, and preparing weekly progress reports. The role also involves troubleshooting experimental procedures, optimizing laboratory protocols, assisting with ordering supplies, and maintaining laboratory organization. Adherence to institutional laboratory safety procedures and participation in laboratory meetings are also key aspects of this role.

Requirements

Strong organizational skills and attention to detail.
Excellent written documentation and scientific communication skills.
Ability to work independently while collaborating effectively with laboratory members.
Strong problem-solving skills and willingness to optimize and improve experimental protocols.
Master's degree in Biology, Genetics, Molecular Biology, Cell Biology, Biochemistry, or a related discipline.
Experience with the molecular techniques described above.
Hands-on experience working with Drosophila melanogaster.

Responsibilities

Maintains and manages Drosophila melanogaster stocks, including setting genetic crosses, collecting progeny, and performing routine husbandry.
Prepares and processes Drosophila tissues for downstream analyses.
Performs immunofluorescence staining on whole-mount tissues.
Conducts whole-mount in situ hybridization experiments.
Operates laser-scanning confocal microscopy systems to acquire high-quality fluorescence images.
Processes and analyzes confocal images using appropriate image analysis software.
Performs RNA isolation from Drosophila tissues.
Carries out reverse transcription quantitative PCR (RT-qPCR), including sample preparation, assay setup, and data analysis.
Prepares and analyzes polytene chromosome squashes.
Maintains detailed laboratory notebooks and electronic records.
Prepares concise weekly written progress reports summarizing experimental results, troubleshooting efforts, and future plans.
Troubleshoots existing experimental procedures and systematically optimize laboratory protocols to improve reproducibility, efficiency, and data quality.
Assists with ordering laboratory supplies and maintaining laboratory organization.
Follows institutional laboratory safety procedures and maintain compliance with applicable regulations.
Participates in laboratory meetings and contribute to discussions of experimental design and data interpretation.