Post Doctoral Scholar

Oregon Health & Science University • Portland, OR

9d • Onsite

About The Position

The OHSU Dermatology Research Division merges basic, clinical and translational research in epithelial cell cancers and dermatologic diseases. Our basic and clinician-scientists are committed to innovative research on cancer and inflammation of skin with an impact on understanding causes and improving future treatment of muco-cutaneous diseases and providing more effective patient care. The person who fills this position will report to Noah Hornick, MD, PhD, Assistant Professor, in investigating methods for surveillance of melanoma recurrence / metastasis, as well as mechanisms of inflammation in the skin during adverse reactions to cancer immunotherapy. Responsibilities will include conducting experimentation that leverages techniques including the processing of human tissue samples (skin, tumor, and blood) in order to extract single cells, nuclei, DNA, RNA, and protein; histologic work including the chromogenic and fluorescent labeling and imaging of slides, preparation of spatial, single-cell, and bulk RNA libraries for sequencing, cell culture, and flow cytometry. The Research Associate will also be responsible for contributing to documentation and data analysis related to this experimentation, as well as to the preparation of figures for publication. Function/Duties of Position Supports basic day-to-day functions of the lab, including assisting with ordering supplies. Oversees and assists with training of new or temporary students and other personnel. Attends all laboratory research and division/department meetings. Independently sets up and carries out cell culture experiments with cell lines and primary human skin cells. Analyzes results using techniques listed in position summary. Independently prepares, labels, and images slides using stains, immunolabeling, and in situ hybridization with both chromogens and fluorophores. Processes human blood and tissue to produce single-cell suspensions, single-nucleus suspensions, peripheral blood mononuclear cells, serum, and plasma. Prepares cDNA libraries for sequencing from fixed and frozen tissue through 10X Genomics protocols: Visium, Chromium, Chromium Flex, and Epi Multiome. Prepares and labels single-cell suspensions from patient samples and cultured cells, performs flow cytometry analysis.

Requirements

• PhD in related field (molecular, cell biology, or biochemistry)
• Will vary depending on years of postdoctoral training completed
• Fluency with relevant mathematics / basic statistics
• Experience with MS Excel
• Biochemistry and molecular biology research techniques
• Tissue culture skill

Nice To Haves

• Experience or training in bioinformatics helpful
• Prior imaging or histology experience
• Single-cell transcriptome analysis experience
• R programming experience
• Python programming experience
• Knowledge of immunology
• Knowledge of dermatology
• Knowledge of sex hormone biology

Responsibilities

• Supports basic day-to-day functions of the lab, including assisting with ordering supplies.
• Oversees and assists with training of new or temporary students and other personnel.
• Attends all laboratory research and division/department meetings.
• Independently sets up and carries out cell culture experiments with cell lines and primary human skin cells.
• Analyzes results using techniques listed in position summary.
• Independently prepares, labels, and images slides using stains, immunolabeling, and in situ hybridization with both chromogens and fluorophores.
• Processes human blood and tissue to produce single-cell suspensions, single-nucleus suspensions, peripheral blood mononuclear cells, serum, and plasma.
• Prepares cDNA libraries for sequencing from fixed and frozen tissue through 10X Genomics protocols: Visium, Chromium, Chromium Flex, and Epi Multiome.
• Prepares and labels single-cell suspensions from patient samples and cultured cells, performs flow cytometry analysis.
• Contributes to documentation and data analysis related to experimentation.
• Contributes to the preparation of figures for publication.

Benefits

• Opportunities to learn and advance

What This Job Offers

• Job Type: Full-time
• Career Level: Entry Level
• Education Level: Ph.D. or professional degree