Post-Doctoral Research Associate: Department of Biochemistry & Cellular and Molecular Biology - UTK

University of Tennessee•Knoxville, TN

21d•$55,000 - $60,000•Onsite

About The Position

The Joshi lab is seeking a highly motivated postdoctoral candidate to investigate the biogenesis of membrane-bound organelles, specifically lipid droplets and peroxisomes. The research will focus on understanding the molecular mechanisms by which these organelles form from the ER membrane, utilizing budding yeast and mammalian cell culture systems. The project involves characterizing ER subdomains, identifying their protein and lipid composition, and studying membrane curvature induced by ER shaping proteins like Pex30 (yeasts) and MCTPs (mammals) using Cryo-EM. The lab employs techniques such as yeast and mammalian cell culture, confocal and super-resolution microscopy, live-cell imaging, advanced image acquisition and quantitation, and various biochemical and molecular biology techniques. This is a grant-funded position contingent upon continued grant funding.

Requirements

Highly motivated postdoctoral candidate.
Experience with budding yeast and mammalian cell culture systems.
Experience with confocal and super-resolution microscopy.
Experience with live-cell imaging.
Experience with advanced image acquisition and quantitation methods.
Experience with biochemical and molecular biology techniques.
Experience with Cryo-EM.

Responsibilities

Study the biogenesis of membrane-bound organelles such as lipid droplets and peroxisomes.
Understand the molecular mechanisms of organelle biogenesis from the ER membrane.
Characterize ER subdomains to identify their protein and lipid composition.
Study membrane curvature induced by ER shaping proteins using Cryo-EM.
Utilize yeast and mammalian cell culture systems, confocal and super-resolution microscopy, live-cell imaging, advanced image acquisition and quantitation methods, and biochemical and molecular biology techniques.