Post Doctoral Fellow-MSH-30001-002
About The Position
This project aims to develop and investigate human cortical brain organoids (hCBOs) derived from induced pluripotent stem cells (iPSCs) to elucidate molecular mechanisms and assess potential treatment strategies for PDHA1-related pyruvate dehydrogenase complex (PDHc) deficiency. The study will integrate structural, metabolic, and functional assessments to identify disease-related alterations and evaluate therapeutic interventions. The first three months will focus on generating hCBOs from patient-derived and control iPSCs. From months four to six, neuronal structure and development will be characterized at the molecular level. Single-cell RNA sequencing (scRNA-seq) will be performed to identify cell-type-specific transcriptional alterations in PDHA1-deficient organoids. Parallel metabolic analyses will begin, using metabolomic and proteomic profiling to determine how PDHA1 deficiency affect glycolysis, oxidative phosphorylation, and overall metabolic function of hCBOs.
Requirements
- PhD or equivalent degree in a relevant scientific field.
- Experience with induced pluripotent stem cells (iPSCs).
- Experience with human cortical brain organoid (hCBO) generation and culture.
- Proficiency in molecular biology techniques.
- Experience with single-cell RNA sequencing (scRNA-seq).
- Experience with metabolomic and proteomic profiling.
- Strong analytical and problem-solving skills.
- Excellent written and verbal communication skills.
Nice To Haves
- Experience in neurodegenerative disease research.
- Familiarity with PDHA1-related PDHc deficiency.
- Experience with structural, metabolic, and functional assessments of organoids.
Responsibilities
- Develop and investigate human cortical brain organoids (hCBOs) derived from induced pluripotent stem cells (iPSCs).
- Elucidate molecular mechanisms and assess potential treatment strategies for PDHA1-related pyruvate dehydrogenase complex (PDHc) deficiency.
- Integrate structural, metabolic, and functional assessments to identify disease-related alterations and evaluate therapeutic interventions.
- Generate hCBOs from patient-derived and control iPSCs.
- Characterize neuronal structure and development at the molecular level.
- Perform single-cell RNA sequencing (scRNA-seq) to identify cell-type-specific transcriptional alterations.
- Conduct metabolic analyses, including metabolomic and proteomic profiling.
Benefits
- Commitment to fostering an environment where everyone can contribute to excellence.
- Unparalleled legacy of achievement, education, and innovation.
- Encouragement to actively participate in creating a culture that ensures fair access to opportunities, promotes inclusive practices, and supports the success of every individual.
- Leaders committed to fostering a workplace where all employees feel valued, respected, and empowered to grow.
- Environment where collaboration, fairness, and continuous learning drive positive change.
- Supportive and welcoming environment where everyone has the opportunity to thrive and advance professionally.
Stand Out From the Crowd
Upload your resume and get instant feedback on how well it matches this job.
What This Job Offers
Job Type
Full-time
Career Level
Entry Level
Education Level
Ph.D. or professional degree
