Post Doctoral Fellow

Mass General Brigham • Boston, MA

6h • Onsite

About The Position

Postdoctoral position available to study mechanisms of vascular biology, lung injury and lung fibrosis in the Division of Pulmonary and Critical Care Medicine at Massachusetts General Hospital and Harvard Medical School. The Knipe Lab is looking for a hard-working and enthusiastic post-doctoral candidate to work on a funded project investigating mechanisms of endothelial cell dysfunction in the context of fibrosis in the lungs. We are interested in strategies to improve endothelial function in the context of fibrotic lung diseases like scleroderma associated ILD and idiopathic pulmonary fibrosis (IPF). This project involved both animal modeling of lung disease (mice) and in vitro cell culture work with human microvascular endothelial cells.

Requirements

• Ph.D. in Molecular Biology, Cellular Biology, or a related discipline.
• 0-1 year of relevant post-graduate experience.
• Hands-on expertise in molecular biology.
• Hands-on expertise in in vivo disease models.
• Track record of significant contributions to research projects and scientific publications.
• Ability to work independently.
• Ability to work as part of a highly collaborative team.
• Mouse handling, husbandry and disease modeling including dissection and tissue harvesting.
• Aseptic technique and in vitro culture of mammalian cells.
• Proficiency with molecular biology techniques including western blotting and qRT-PCR.

Nice To Haves

• Experience with multiple routes of drug delivery.
• Experience with primary cell isolation.
• Experience with 3D cultures.
• Exposure to basics of histology and microscopy including widefield and confocal imaging.
• Exposure to flow cytometry and cell sorting.
• Exposure to large data set analysis and/or bioinformatics.
• Prior post-doctoral experience.

Responsibilities

• Design, execute, analyze and document in vitro and in vivo studies to define the role of endothelial dysfunction and vascular permeability in the development of fibrosis.
• Conduct research in vascular biology, lung injury, and lung fibrosis.
• Investigate mechanisms of endothelial cell dysfunction in the context of lung fibrosis.
• Explore strategies to improve endothelial function in fibrotic lung diseases.
• Perform animal modeling of lung disease (mice).
• Conduct in vitro cell culture work with human microvascular endothelial cells.
• Generate high impact publications and presentations at conferences.
• Prepare for a long-term career in academia or industry.

What This Job Offers

• Job Type: Full-time
• Career Level: Entry Level
• Education Level: Ph.D. or professional degree
• Number of Employees: 5,001-10,000 employees