Post Doctoral Associate

University of Pittsburgh • Pittsburgh, PA

3d • Onsite

About The Position

The Department of Biological Sciences at the University of Pittsburgh invites applications for a postdoctoral associate in molecular biology/genomics, to begin as soon as possible. The successful candidate will work with members of the Kaplan laboratory to create mammalian cell models for human disease alleles using CRISPR/Cas9 editing and genomic/deep sequencing analysis of model alleles. Projects include the establishment of a Multiplexed Assay of Variant Effects for an essential gene linked to neurodevelopmental syndrome and characterization of individually edited human cell lines using genomic approaches. The Department of Biological Sciences is a highly interactive community situated on the Oakland campus of the University of Pittsburgh. We are dedicated to the mutual success of our faculty and students in our research, education, and outreach missions. The department and university nurture a strong teaching community with active efforts in teaching innovation and pedagogical research. Pittsburgh is a city that is often voted "most livable" in the nation. Further information about the Department of Biological Sciences is available at: http://www.biology.pitt.edu.

Requirements

• PhD in biology or applicable field.
• Background in mammalian cell culture.
• Background in molecular mechanisms.
• Mammalian cell culture expertise.
• Experience or familiarity with CRISPR/Cas9-mediated genome editing.

Nice To Haves

• Experience with growing and differentiating iPSCs.
• Experience with genomic library construction.
• Experience with molecular biology and protein analysis.
• Familiarity with bioinformatic analysis of genomic data.

Responsibilities

• Create mammalian cell models for human disease alleles using CRISPR/Cas9 editing.
• Perform genomic/deep sequencing analysis of model alleles.
• Establish a Multiplexed Assay of Variant Effects for an essential gene linked to neurodevelopmental syndrome.
• Characterize individually edited human cell lines using genomic approaches.

What This Job Offers

• Job Type: Full-time
• Career Level: Entry Level
• Education Level: Ph.D. or professional degree