Laboratory Technician II-Department of Neurosurgery-RNA Lab

University of Florida•Gainesville, FL

1d•$22 - $25•Onsite

About The Position

Under general supervision, this role involves performing human clinical trial biospecimen processing, including PBMC isolation, serum processing, CTC processing, and tissue banking. The technician will also be involved in clinical immune monitoring and research support, including sample preparation for flow cytometry and RNA sequencing. Additionally, the role requires maintaining laboratory operations, compliance with safety standards, and providing administrative and departmental support. Training and professional development are also key aspects of this position.

Requirements

Associate's degree in an appropriate area and two years of related experience; or a high school diploma or equivalent and four years of related experience.
Appropriate college course work or vocational/technical training may substitute at an equivalent rate for the required experience.

Nice To Haves

Masters Degree and experience with Clinical patient sample processing and banking
Some Experience in flow cytometry, Serum Elisa and other clinical assays.

Responsibilities

Perform human clinical trial biospecimen processing, including peripheral blood mononuclear cell (PBMC) isolation, processing, and cryopreservation, serum processing, CTC processing and tissue banking for all clinical biopsies from patients.
Process, label, store, and bank clinical research samples according to study protocols and laboratory procedures.
Maintain accurate electronic and paper records related to sample collection, processing, storage, and shipment.
Organize and track clinical specimens to ensure proper chain of custody and sample integrity.
Assist with maintaining clinical trial documentation and study records.
Monitor inventory levels and help order supplies required for clinical sample processing activities.
Follow established SOPs and assist with SOP updates as directed.
Assist with clinical immune-monitoring studies using patient-derived samples.
Prepare and stain samples for flow cytometry analysis.
Operate laboratory instrumentation, including flow cytometers and related equipment, following established protocols.
Process patient PBMCs for downstream applications, including singlecell RNA sequencing and bulk RNA analysis.
Perform RNA extraction and other molecular biology procedures according to established methods.
Assist with confocal microscopy experiments and routine laboratory assays.
Record experimental results and maintain laboratory notebooks and electronic data files.
Update Electronic Teams notebooks and onedrive files with the raw and processed/graphed data results.
Assist in data compilation, quality control reviews, and preparation of research reports and presentations.
Prepare laboratory reagents, buffers, and materials for research clinical immune monitoring activities.
Maintain inventory of all supplies for assays related to immune monitoring as well as records and stock laboratory supplies.
Ensure laboratory workspaces remain organized and compliant with laboratory safety standards.
Participate in biosafety, chemical safety, and regulatory compliance activities.
Assist with maintaining laboratory clinical batch records and documentation required for inspections and and FDA/NIH audits.
Maintain the clinical trial notebooks and the electronic teams folders for all clinical processing of samples and keep all records, images and locations of clinical samples up to date weekly.
Maintain the electronic spreadsheets of Clinical patient samples, organized by sample type, (PBMC, Serum, Cell free DNA/RNA, CTC and tissue Biopsies) make sure all samples are recorded and logged at intake during processing and when removed for immune monitoring assays.
Attend laboratory meetings, seminars, and training sessions.
Participate in continuing education opportunities to maintain technical competency.
Assist with training new laboratory personnel on routine laboratory procedures as directed.
Provide support for laboratory inspections, audits, and accreditation activities.
Assist with preparation of routine laboratory reports, meeting materials, and other administrative documents.
Participate in departmental activities and collaborative projects as assigned.