Laboratory Technician II

University of Florida•Gainesville, FL

4d•Onsite

About The Position

The Laboratory Technician II will participate in various animal studies, including administering pharmacological agents and immunotherapy via different injection methods, and assisting with imaging techniques such as MRI, CT, and MPI. The role involves experimental approaches using magnetic nanoparticles and plays a key role in regulatory management, specifically with IACUCs. Responsibilities also include histological work such as collecting and preparing tissues, preparing slides, staining, and conducting image capture and analysis. Molecular techniques include molecular cloning, plasmid purification, primer design, RNA and DNA isolation, real-time PCR, PCR genotyping, immunoassays, cell line maintenance, and protein analysis via western blots. The technician will also perform multiparameter flow cytometry and develop multicolor flow panels. A significant part of the role involves ensuring laboratory compliance with governing rules and regulations, coordinating IACUC and IRB submissions, meeting EH&S standards, and training new laboratory members. Additionally, the technician will train and supervise students performing diagnostic procedures related to immune therapy experiments and perform other duties as needed to fulfill the mission and abide by the values of the College of Medicine, including performing tasks and experiments as directed by the Principal Investigator.

Requirements

Associate's degree in an appropriate area and two years of related experience; or a high school diploma or equivalent and four years of related experience.
Appropriate college course work or vocational/technical training may substitute at an equivalent rate for the required experience.
Working knowledge of basic laboratory techniques.
Must be extremely knowledgeable in laboratory procedures and must be meticulous in recording, tracking & reporting of data collected.

Nice To Haves

Animal colony management and experience handling rodents (highly preferred).
Experience administering treatments i.v., i.p., i.m., and/or intracranially to rodents (highly preferred).
Experience maintaining cell lines, performing plasmid transfections/viral infections, immunohistochemistry, FACS, rt-PCR, genotyping, immunohistochemistry, western blotting, microscopy.
Advanced degree in related scientific field.
Strong interest in translational cancer research.

Responsibilities

Participating in treating animals with pharmacological agents or immunotherapy either through i.p., i.m., i.v., or intracranial injection.
Assisting with experimental approaches using magnetic nanoparticles.
Collecting organs and other tissues and preparing for histopathology.
Preparing slides, staining with standard histology stains such as H&E, as well as with immunohistochemistry and immunofluorescence.
Conducting image capture with fluorescent microscope, confocal microscope, multiphoton confocal microscope and analysis of tissue following intervention studies.
Performing molecular cloning, plasmid purification, primer design, RNA and DNA isolation.
Isolating RNA and performing real-time PCR, and PCR genotyping of transgenic mouse lines.
Performing immunoassays to gene therapy vectors and transgenes, including ELISAs.
Performing protein analysis by isolating protein of interest from cell or tissue lysates and performing/analyzing western blots.
Perform multiparameter flow cytometry and develop proficiency setting multicolor flow panels.
Ensuring the laboratory is compliant with governing rules and regulations.
Coordinate all IACUC and IRB (exempt) submissions/renewals.
Ensure all EH&S standards are met.
Train new laboratory members to ensure compliance standards are met.
Train and supervise graduate and undergraduate students performing diagnostic procedures related to immune therapy experiments.
Performing tasks and experiments as directed by the Principal Investigator.