Laboratory Technician II
About The Position
The Laboratory Technician II will participate in various research activities within the College of Medicine. This role involves animal studies, including administering pharmacological agents and immunotherapy, assisting with imaging techniques (MRI, CT, MPI), and working with magnetic nanoparticles. The position also requires performing histological work such as tissue collection, slide preparation, staining (H&E, immunohistochemistry, immunofluorescence), and image analysis. Molecular techniques include molecular cloning, plasmid purification, RNA/DNA isolation, real-time PCR, PCR genotyping, immunoassays, protein analysis (western blots), and multiparameter flow cytometry. The role also entails ensuring regulatory compliance with IACUC and IRB submissions, adhering to EH&S standards, and training new laboratory members and students. The technician will also train and supervise graduate and undergraduate students in diagnostic procedures related to immunotherapy experiments and perform other duties as needed to support the Principal Investigator's research.
Requirements
- Associate's degree in an appropriate area and two years of related experience; or a high school diploma or equivalent and four years of related experience.
- Appropriate college course work or vocational/technical training may substitute at an equivalent rate for the required experience.
Nice To Haves
- Animal colony management and experience handling rodents (highly preferred).
- Experience administering treatments i.v., i.p., i.m., and/or intracranially to rodents (highly preferred).
- Experience maintaining cell lines, performing plasmid transfections/viral infections, immunohistochemistry, FACS, rt-PCR, genotyping, immunohistochemistry, western blotting, microscopy
- Advanced degree in related scientific field.
- Working knowledge of basic laboratory techniques.
- Must be extremely knowledgeable in laboratory procedures and must be meticulous in recording, tracking & reporting of data collected.
- Strong interest in translational cancer research
Responsibilities
- Participating in treating animals with pharmacological agents or immunotherapy either through i.p., i.m., i.v., or intracranial injection.
- Assisting with experimental approaches using magnetic nanoparticles.
- Collecting organs and other tissues and preparing for histopathology.
- Preparing slides, staining with standard histology stains such as H&E, as well as with immunohistochemistry and immunofluorescence.
- Conducting image capture with fluorescent microscope, confocal microscope, multiphoton confocal microscope and analysis of tissue following intervention studies.
- Performing molecular cloning, plasmid purification, primer design, RNA and DNA isolation.
- Isolating RNA and performing real-time PCR, and PCR genotyping of transgenic mouse lines.
- Performing immunoassays to gene therapy vectors and transgenes, including ELISAs.
- Performing protein analysis by isolating protein of interest from cell or tissue lysates and performing/analyzing western blots.
- Performing multiparameter flow cytometry and developing proficiency setting multicolor flow panels.
- Ensuring the laboratory is compliant with governing rules and regulations.
- Coordinating all IACUC and IRB (exempt) submissions/renewals.
- Ensuring all EH&S standards are met.
- Training new laboratory members to ensure compliance standards are met.
- Training and supervising graduate and undergraduate students performing diagnostic procedures related to immune therapy experiments.
- Performing tasks and experiments as directed by the Principal Investigator.
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What This Job Offers
Career Level
Entry Level
Education Level
Associate degree
