Laboratory Sciences Professional ( Open Rank: Entry – Intermediate)

University of Colorado•Aurora, CO

2d•Onsite

About The Position

This is a full-time (40 hours per week), open rank (Entry – Intermediate level) Research Services Laboratory Position at the Barbara Davis Center (BDC) for Diabetes. The successful candidate will work in the Research Division of the BDC in the laboratory of Dr. Lori Sussel on projects exploring the molecular mechanisms regulating pancreatic islet development and function in normal and diabetic conditions. The laboratory primarily uses cell lines and genetically modified mice as model systems for molecular studies. The successful candidate will play an important role in identifying novel mechanisms underlying islet cell dysfunction in T1D and T2D.

Requirements

Bachelor’s degree in biology, molecular biology, chemistry, genetics or related field
Specific work experience in molecular, biochemical or cell biology techniques.
Mouse handling experience
One (1) year professional level research experience
Applicants must meet minimum qualifications at the time of hire.
A combination of education and related technical/military/paraprofessional experience may be substituted for a bachelor’s degree on a year for year basis.

Nice To Haves

Specific work experience in molecular, biochemical or cell biology techniques.
Mouse handling experience
Computer literate with proficiency in Microsoft Office Software programs.
Experience with Data Science
Two (2) years of experience in an experimental or wet laboratory
Knowledge of basic laboratory techniques including safety procedures and techniques
Knowledge of human stem cell research techniques
Knowledge of mouse pancreas research techniques
Ability to analyze immunofluorescent data
Ability to communicate effectively, both in writing and orally
Ability to establish and maintain effective working relationships with employees at all levels throughout the institution
Outstanding customer service skills
Demonstrated commitment and leadership ability to advance diversity and inclusion
Knowledge of basic human anatomy, physiology medical terminology
Ability to interpret and master complex research protocol information
Willing to work with cells, tissues and laboratory mice

Responsibilities

Collaborate with and support Principal Investigator (PI) and lab members with laboratory and research functions
Utilize a variety of laboratory techniques required for research projects
Perform experiments and tabulate and analyze research
Maintenance of a laboratory inventory.
Ordering and restocking reagents for the laboratory.
Maintenance and genotyping of mouse colonies.
Maintenance of mouse and human cell cultures.
Molecular cloning
Molecular and histological analysis of mouse tissues, including genomic DNA isolation, PCR, RNA isolation, gene expression analysis by qPCR and NGS sequencing, protein localization or quantification by immunofluorescence analysis, Western blotting, or ELISA assay.
In vitro knockdown experiments using siRNA followed by quantitative analysis of gene expression.
Assist in the review and implementation of lab support policies, practices and programs to meet organizational, operations and management needs
Assist with ensuring compliance with applicable laws, rules and regulations; receive guidance and direction or escalate when required