Lab Technician I - Laboratory
About The Position
Medical Diagnostic Laboratories (MDL), a member of Genesis Biotechnology Group, is a CLIA-certified clinical laboratory with multiple state licenses. MDL specializes in automated DNA-based molecular analysis and PCR testing, with research and testing in areas including gynecology, infectious diseases, infectious arthritis, tick-borne diseases, mycology, and chronic fatigue syndrome. The Laboratory Technician performs specimen accessioning and a range of laboratory procedures involving extraction, PCR, gel electrophoresis, pyrosequencing, microbiology and cell culture, and next-generation sequencing. The role also includes maintaining safe handling of materials, protecting customer privacy, contributing as a team member, communicating information clearly and promptly, taking initiative to resolve problems, and maintaining productive work habits, accuracy, and quality. The position is in a laboratory setting and may involve use of personal protective equipment and exposure to chemicals and blood or body fluids.
Requirements
- Complete 9-month Clinical Laboratory Technician training.
- Bachelor's degree in Biology, Chemistry, or a related science field.
- Ability to sit, stand, and walk, and to perform repetitive manual pipetting.
- Proficient visual and audio skills.
Responsibilities
- Print labels and attach them to requisitions and corresponding specimens; accession multiple specimen types; photograph received specimens; identify discrepancies; file paperwork and distribute it to QC bins; scan requisitions and 2-D barcode elution tubes; and locate and record temperatures for required stations.
- Perform manual phenol/chloroform extractions on biopsies and ticks, separate organic and inorganic layers, and perform quantitative analysis and hydration of ticks and biopsies.
- Extract samples using robotic systems and an XG sheet template; set up liquid-handling robotics with appropriate reagent volumes; dispose of hazardous and non-hazardous waste; address robotic user interactions or malfunctions; and manually extract samples using commercial kits.
- Perform manual and robotic RNA extractions using Qiagen and VX Reagents kits as required.
- Perform manual and robotic conventional and real-time PCR amplification; load and start thermocycler equipment; conduct initial analyses of completed real-time PCR; perform Acoustic Real-Time PCR using an ATS-100 Acoustic Dispenser; and analyze completed acoustic real-time PCR.
- Determine which completed PCRs require gel electrophoresis; select or prepare the appropriate 2% or 2.5% gels; complete blank gel template sheets; stain samples; load gels with appropriate ladder and samples; select run settings; capture gel images using a SYNGENE UV gel reader; and troubleshoot gel box, power source, or gel reader errors.
- Perform nested PCR before sequencing; load and run Sequencing Analysis and Single Nucleotide Polymorphism reactions; and analyze run data.
- Inoculate appropriate microbiology plates with swab media and streak for single colonies; identify species and inoculate appropriate antibiotic plates; analyze growth for antibiotic susceptibility or resistance; operate the Anaerobic Chamber System for GBS antibiotic resistance testing; identify and pick colonies for PCR; and perform and analyze CAMP tests for hemolysis zones.
- Perform nested PCRs for BRCA, HPV, and cystic fibrosis; attach samples to bead mixtures; clean beads to remove unused dNTPs and primers; perform gel purification using a Pippin Prep machine; operate Life Technologies equipment; and analyze run data.
- Perform other similar or related duties as assigned.
- Conduct activities safely and handle materials according to safety guidelines; maintain customer privacy; participate as a team member and remain accountable for assigned work; communicate information in a supportive, timely, and understandable manner; proactively diagnose and resolve problems; and maintain productive work habits, accuracy, and quality.
Benefits
- Competitive salary
- Medical, dental, and vision insurance
- Short- and long-term disability
- Life insurance
- Dependent care flexible spending account
- Voluntary policies: accident, hospital indemnity, critical illness, and supplemental short-term disability
- Identity theft protection
- 401(k) plan
- Paid vacation and holidays
- Business casual environment
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What This Job Offers
Job Type
Full-time
Career Level
Entry Level
