Lab Tech

University of Pennsylvania

2d•$7 - $12•Onsite

About The Position

The University of Pennsylvania, the largest private employer in Philadelphia, is a world-renowned leader in education, research, and innovation. This historic, Ivy League school consistently ranks among the top 10 universities in the annual U.S. News & World Report survey. Penn has 12 highly-regarded schools that provide opportunities for undergraduate, graduate and continuing education, all influenced by Penn’s distinctive interdisciplinary approach to scholarship and learning. As an employer Penn has been consistently ranked nationally on many occasions with the most recent award from Forbes who named Penn one of America’s Best Large Employers. Penn offers a unique working environment within the city of Philadelphia. The University is situated on a beautiful urban campus, with easy access to a range of educational, cultural, and recreational activities. With its historical significance and landmarks, lively cultural offerings, and wide variety of atmospheres, Philadelphia is the perfect place to call home for work and play. Generate and maintain mouse models of open angle glaucoma including long term maintenance of a mouse colony; conduct visual functional testing (e.g. optokinetic response and pattern ERG). The PI will train the successful applicant in methods of model generation. Dissect, process, mount/embed, cryosection and immunohistochemical staining of relevant ocular specimens and tissues; imagining and subsequent analysis of stained tissues; image capturing with microscope including live imagining microscopes. Isolation of protein, RNA, and DNA from cultured cells and isolated murine ocular tissues with standard methods for western blot, PCR, sequencing, and ELISA assays. Statistical and image analyses with various programs including Prism; Image J; Adobe Photoshop and Illustrator; Microsoft Excel; and generating presentation slides using Microsoft PowerPoint.

Requirements

Basic understanding of laboratory techniques
Ability to follow detailed instructions
Good organizational skills
Ability to maintain accurate records
Proficiency in Microsoft Office Suite (Word, Excel, PowerPoint)
Experience with statistical analysis software (e.g., Prism) is a plus
Experience with image analysis software (e.g., Image J, Adobe Photoshop, Illustrator) is a plus

Nice To Haves

Experience with mouse colony maintenance
Experience with immunohistochemical staining
Experience with protein, RNA, and DNA isolation
Experience with western blot, PCR, sequencing, and ELISA assays

Responsibilities

Generate and maintain mouse models of open angle glaucoma including long term maintenance of a mouse colony
Conduct visual functional testing (e.g. optokinetic response and pattern ERG)
Dissect, process, mount/embed, cryosection and immunohistochemical staining of relevant ocular specimens and tissues
Imaging and subsequent analysis of stained tissues
Image capturing with microscope including live imagining microscopes
Isolation of protein, RNA, and DNA from cultured cells and isolated murine ocular tissues with standard methods for western blot, PCR, sequencing, and ELISA assays
Statistical and image analyses with various programs including Prism; Image J; Adobe Photoshop and Illustrator; Microsoft Excel
Generating presentation slides using Microsoft PowerPoint