Intern, Biophysics & Protein Sciences Group

Revolution Medicines•Redwood City, CA

2d•Onsite

About The Position

Revolution Medicines is a late-stage clinical oncology company developing novel targeted therapies for patients with RAS-addicted cancers. The company’s R&D pipeline comprises RAS(ON) inhibitors designed to suppress diverse oncogenic variants of RAS proteins. The company’s RAS(ON) inhibitors daraxonrasib (RMC-6236), a RAS(ON) multi-selective inhibitor; elironrasib (RMC-6291), a RAS(ON) G12C-selective inhibitor; zoldonrasib (RMC-9805), a RAS(ON) G12D-selective inhibitor; and RMC-5127, a RAS(ON) G12V-selective inhibitor, are currently in clinical development. As a new member of the Revolution Medicines team, you will join other outstanding professionals in a tireless commitment to patients with cancers harboring mutations in the RAS signaling pathway. The Opportunity: The Biophysics and Protein Sciences Group generates target proteins to support drug discovery efforts. We currently utilize heterologous overexpression systems, including bacterial and insect cell expression, and purify proteins using a variety of approaches (e.g., bulk purification, FPLC, immunoprecipitation). To maximize our capacity to test multiple construct designs and rapidly determine which proteins can be reliably produced, we are actively developing a small-scale expression and purification (SmScExpPur) platform along with a series of protocols spanning the full workflow: from construct design through small-scale expression testing, purification, and quality control. The summer intern will contribute to this ongoing effort by owning a defined set of experiments aimed at further developing and evaluating SmScExpPur protocols, with the goal of improving protein yield, consistency, and quality. This will include executing small-scale expression and purification experiments, analyzing outcomes across constructs and conditions, and contributing data-driven recommendations for protocol improvements. Proteins generated through this work will be used to perform basic QC assessments to evaluate protein identity, aggregation state, and folding.

Requirements

Prior hands-on laboratory experience in an academic or industry setting.
Strong attention to detail and experimental rigor.
Clear written and verbal communication skills.
Experience with basic laboratory techniques, including pipetting.
Experience documenting experiments through protocols, laboratory notebooks, laboratory reports, or research papers.
Familiarity with bacterial transformation and/or recombinant protein expression workflows.

Nice To Haves

Experience with insect cell expression systems.
Experience with protein purification techniques (e.g., affinity purification, FPLC).
Interest or experience in protocol development, optimization, or troubleshooting.
Experience with liquid handling or semi-automated laboratory systems.

Responsibilities

Executing small-scale expression and purification experiments.
Analyzing outcomes across constructs and conditions.
Contributing data-driven recommendations for protocol improvements.
Performing basic QC assessments to evaluate protein identity, aggregation state, and folding.