In Vivo Scientist

Eurofins•Upper Gwynedd Township, PA

9h•Onsite

About The Position

We are seeking a highly motivated In Vivo Scientist to contribute to discovery research. This position will focus on HIV research, including virus production, characterization, and support of HIV discovery efforts.

Requirements

Ph.D. in Biomedical Sciences, Microbiology, Immunology, Virology, or a related scientific discipline. Equivalent combination of a lesser degree and industry experience would also be acceptable.
Demonstrated experience in HIV research and BSL-2 laboratory operations, with a strong HIV publication record preferred.
Familiarity with HIV host-pathogen interactions.
Expertise in viral propagation, concentration, and characterization techniques, including ultracentrifugation, viral titer determination, and neutralization assays.
Hands-on experience with molecular biology techniques, including CRISPR gene editing, siRNA-mediated knockdown, transfection, site-directed mutagenesis, and Western blotting.
Experience with in vivo mouse studies, including animal handling, tissue harvesting, and isolating viral RNA from ex vivo tissues and/or blood samples.
Experience with ELISA.
Proficiency with scientific data analysis and visualization software, including FlowJo, GraphPad Prism, and SnapGene or Geneious Prime.
Strong problem-solving skills, attention to detail, and the ability to work effectively in a collaborative research environment.
Authorization to work in the United States indefinitely without restrictions or sponsorship.

Nice To Haves

Willingness to learn omics techniques, i.e. MSD multiplex assays, Nomics, Olink, 10X Genomics scRNAseq.

Responsibilities

Lead the design, execution, optimization, and characterization of HIV production workflows. As well as inventory management.
Conduct HIV discovery research using primary human cells (PBMCs, CD4+ T cells) and immortalized cell lines (THP-1, Jurkat, MT-4).
Perform and analyze experiments utilizing multi-parameter flow cytometry (2 colors or more), RT-qPCR, PCR, ELISA, and MSD multiplex assays.
Generate and characterize stable cell lines expressing proteins of interest.
Support in vivo studies, including tissue collection and processing, and perform ex vivo viral RNA isolation from tissues and/or blood samples.
Analyze and interpret experimental data, communicate findings, and contribute to project strategy and execution.