Flow Cytometry Technician I

Seattle Children's Hospital•Seattle, WA

3d•Onsite

About The Position

This role is responsible for the operation and maintenance of flow cytometry instruments within a core facility. The technician will perform daily quality control, preventative maintenance, and troubleshooting for a variety of analytical and cell sorting instruments. They will also ensure adherence to Standard Operating Procedures (SOPs) and safety protocols, including Biosafety Level 2 (BSL2+) requirements. The position involves some physical activity such as equipment setup, reagent handling, and lifting up to 50 lbs.

Requirements

Bachelor’s Degree in an Engineering or Scientific discipline.
Experience in a laboratory/research setting.
Demonstrated knowledge in solving technical problems such as issues with lab/flow cytometry instrumentation.

Nice To Haves

Bachelor’s degree in Biology, Immunology, Molecular Biology, Biochemistry, or a related scientific field.
Significant hands-on experience in multi-color flow cytometry, including sample preparation, data acquisition, and data analysis using software such as FlowJo, FCS Express etc.
Demonstrated proficiency in the independent operation, calibration, and maintenance of cell sorters to provide reliable cell sorting services.
Familiarity with standard computer systems, literature search methodologies, and data plotting/statistical analysis.
Prior experience in spectral flow cytometry and imaging technologies is highly preferred.
Excellent verbal and written communication, organizational, and record-keeping skills.
Proven ability to work independently, adaptable team player committed to supporting a collaborative research environment.
Preferred Experience acquiring and analyzing Flow Cytometry data, preferably with BD FacsDiva software.
Data entry and/or word processing experience.
Experience in analyzing data using spreadsheets and statistical analysis.

Responsibilities

Operate and maintain a diverse suite of analytical instruments (including BD Symphony A5, BD LSR Fortessa, Bio-Rad ZE5, and Beckman Coulter Cytoflex) to ensure peak performance and user readiness.
Independently operate, calibrate, and provide expert cell sorting services using advanced cell sorters (e.g., BD S6, Bio-Sorter).
Perform daily QC, preventative maintenance, and tracking for all core instrumentation; maintain detailed, accurate logs and documentation.
Execute first-level instrument troubleshooting to resolve common hardware and software issues.
Escalate complex instrument failures to the Core Leader and coordinate service visits with external vendors.
Ensure strict adherence to Core Standard Operating Procedures (SOPs) and institutional safety protocols, including EH&S and Biosafety Level 2 (BSL2+) regulatory requirements.
Requires some physical activity, including equipment setup, reagent handling, and light lifting (up to 50 lbs.)