Core Rsch Facil Technician III - NextGen DNA Sequencing

University of Florida•Gainesville, FL

7d•$45,000 - $46,000

About The Position

This position performs quality control of samples submitted to the NextGen DNA Sequencing (NGS) lab, assessing their quantity and quality for suitability. The role involves constructing Illumina libraries for sequencing on Illumina platforms, meticulously tracking samples, and documenting laboratory work. Communication with the supervisor and other core personnel regarding experimental results is essential, as is collaboration on defining deliverables and project timelines. The technician will also test, troubleshoot, and implement new protocols for library construction, DNA shearing, sample cleanup, and size selection, staying current with advancements in the field. Contribution to the preparation, updating, and organization of Standard Operating Procedures (SOPs) is also a key responsibility. In addition to technical functions, the role includes general lab operation and maintenance, such as proper biohazard disposal, receiving and storing samples and reagents, monitoring reagent and consumable inventories, and assisting with administrative duties related to receipts and orders. Specific tasks include revision of sample sheets, tracking, and disposal of samples in MyBiotech, as well as freeze and fridge maintenance and documentation in MyBiotech. Other duties as assigned.

Requirements

BA/BS in a related scientific field, or an equivalent combination of education and experience.
Core or technology-specific licensure and/or certifications, where applicable.
Yearly certificate and compliance training, if required.

Nice To Haves

BS/BA in Molecular Biology or a related scientific field.
Familiarity with basic techniques and instrumentation used in the molecular biology laboratory.
Specific NGS procedures including genomic DNA purification, quantification, and manipulation; PCR; NGS DNA library construction and quality assessment/evaluation.
Use of robotics for high-throughput processing of nucleic acids and DNA library samples.
Hands-on experience with Illumina, PacBio, and/or ONT NGS instrumentation and technology.
Basic laboratory citizenship, including organization of equipment and supply inventory.
Instrument run coordination and maintenance.
Preparation of common buffers and solutions.
Computer literacy in Windows/UNIX systems, Microsoft Office package.
Familiarity with utilizing Standard Operating Procedures (SOPs), iLAB, and laboratory information management system (LIMS).

Responsibilities

Performs QC of samples submitted to the NGS lab, assessing quantity and quality for suitability for NextGen DNA sequencing procedures.
Submits samples for qPCR to the Gene Expression core.
Constructs Illumina libraries for sequencing on Illumina platforms.
Properly tracks samples and documents laboratory work.
Communicates with supervisor and other core personnel about experimental results in a timely and professional manner.
Works with the supervisor to define deliverables and timelines for project completion.
Tests, troubleshoots, and implements new protocols for library construction, DNA shearing, sample cleanup, and size selection, using novel procedures.
Contributes to the preparation, updating, and organization of Standard Operating Procedures (SOPs).
Performs maintenance tasks associated with the upkeep of the NGS lab, such as proper disposal of biohazard trash, receiving and storing of samples and reagents.
Monitors reagents and consumables and regularly updates inventories.
Assists with the administrative duties necessary for proper handling of receipts and orders.
Revision of sample sheets, tracking, and disposal of samples in MyBiotech.
Freeze and fridge maintenance and documentation in MyBiotech.