Core Rsch Facil Technician III - NextGen DNA Sequencing

University of Florida • Gainesville, FL

7d

About The Position

This position performs quality control of samples submitted to the NextGen DNA Sequencing (NGS) lab. The technician will assess the quantity and quality of materials to determine their suitability for NGS procedures, submit samples for qPCR, and construct Illumina libraries for sequencing. Responsibilities include tracking samples, documenting laboratory work, communicating experimental results, and collaborating with the supervisor on project deliverables and timelines. The role also involves testing, troubleshooting, and implementing new protocols for library construction, DNA shearing, sample cleanup, and size selection. Additionally, the technician will contribute to the preparation and updating of Standard Operating Procedures (SOPs), perform maintenance tasks for the NGS lab (e.g., biohazard waste disposal, sample and reagent storage), monitor reagents and consumables, update inventories, and assist with administrative duties related to receipts and orders. This includes managing sample sheets, tracking and disposing of samples in MyBiotech, and maintaining freeze/fridge logs in MyBiotech. Other duties as assigned.

Requirements

• BA/BS in a related scientific field, or an equivalent combination of education and experience.
• Core or technology-specific licensure and/or certifications, where applicable.
• Yearly certificate and compliance training, if required.

Nice To Haves

• BS/BA in Molecular Biology or a related scientific field.
• Familiarity with basic techniques and instrumentation used in the molecular biology laboratory.
• Specific NGS procedures including Genomic DNA purification, quantification, and manipulation; PCR; NGS DNA library construction and quality assessment/evaluation.
• Use of robotics for high-throughput processing of nucleic acids and DNA library samples.
• Hands-on experience with Illumina, PacBio, and/or ONT NGS instrumentation and technology.
• Basic laboratory citizenship, including organization of equipment and supply inventory.
• Instrument run coordination and maintenance.
• Preparation of common buffers and solutions.
• Computer literacy in Windows/UNIX systems, Microsoft Office package.
• Familiarity with utilizing Standard Operating Procedures (SOPs), iLAB, and laboratory information management system (LIMS).

Responsibilities

• Performs QC of samples submitted to the NGS lab, assessing quantity and quality for suitability for NextGen DNA sequencing procedures.
• Submits samples for qPCR to the Gene Expression core.
• Constructs Illumina libraries for sequencing on Illumina platforms.
• Properly tracks samples and documents laboratory work.
• Communicates with supervisor and other core personnel about experimental results in a timely and professional manner.
• Works with the supervisor to define deliverables and timelines for project completion.
• Tests, troubleshoots, and implements new protocols for library construction, DNA shearing, sample cleanup, and size selection, using novel procedures.
• Contributes to the preparation, updating, and organization of Standard Operating Procedures (SOPs).
• Performs maintenance tasks associated with the upkeep of the NGS lab, such as proper disposal of biohazard trash, receiving and storing of samples and reagents.
• Monitors reagents and consumables and regularly updates inventories.
• Assists with administrative duties necessary for proper handling of receipts and orders.
• Revision of sample sheets, tracking, and disposal of samples in MyBiotech.
• Freeze and fridge maintenance and documentation in MyBiotech.

What This Job Offers

• Job Type: Full-time
• Career Level: Mid Level