Biological Scientist IV - Pulmonary

Pulmonary•Gainesville, FL

4d•Onsite

About The Position

The Division of Pulmonary, Critical Care & Sleep Medicine is accepting applications for a Biological Scientist IV. The position will reside within the Division’s Alpha-1 Antitrypsin Laboratory and will report directly to Dr. Karina Serban, the laboratory director and an experienced physician-scientist in the field of Alpha-1 deficiency and chronic obstructive pulmonary disease (COPD). This role involves extensive work with human and murine tissue processing, epithelial cell isolation, and precision cut lung slices (PCLS)-related experiments. The scientist will develop novel approaches and analytical tools for harvesting and maintaining cell cultures, design and develop dose escalation studies, and conduct various analyses including immunofluorescence and FACS. Additionally, the position requires intellectual input for troubleshooting organoid and PCLS cultures, scholarly activities related to Alpha-1 Antitrypsin Deficiency and COPD research, data analysis and interpretation for meetings and grants, and participation in bio-product development. The role also involves creating or modifying web-based tools for big data interpretation. Managerial duties include supervising other scientists and students, coordinating experiments with external teams, and preparing performance reports.

Requirements

Bachelor’s degree in biology or a related field and thirteen years of experience or Master’s degree in biology or a related field with eleven year of experience.
Doctoral degree in biology or related field with eight years of experience.

Nice To Haves

Experience with human and murine tissue handling, 3D culture of PCLS and epithelial cells in organoids model are a must.
Independent thinking and the ability to serve as leader on complex projects to investigate new molecular signaling and physiological changes in murine models of Alpha-1 deficiency and COPD.
Expertise in a variety of the field’s concepts, allowing for development of novel practices and procedures.
Ability to work independently with minimal supervision.

Responsibilities

Develop novel approaches and analytical tools as required to harvest and maintain epithelial cells and PCLSs cultures derived from human explanted lungs and murine lungs.
Design and develop dose escalation studies involving alveolar organoids and PCLSs cultures in accordance with IRB and IBC protocols.
Collect, dissect, examine and interpret results of human and murine epithelial cells and tissue harvest.
Develop, organize and conduct immunofluorescence and FACS analysis and data interpretation.
Design and direct metabolism assays (Seahorse), to include oversight of technicians performing testing, review and interpretation of results.
Provide intellectual input as to troubleshooting strategies utilized in organoids and PCLS cultures.
Analyze and interpret data, prepare graphs and figures for meetings and grant submissions.
Present data and manuscripts in weekly lab meeting and annually in divisional meetings.
Design and direct ELISA testing for Alpha-1 Antitrypsin Deficiency to include oversight of technicians performing the testing, review and interpret the results.
Participate in bio-product development to include initial designs, pilot experiments, and collaborating with the Office of Technology and Licensing for assurance of appropriate patents.
Create or modify web-based tools and test potentially new software to provide statistical and computational tools for big data interpretation.
Supervise other scientists and undergraduate students.
Coordinate experiments involving human tissue harvest to include procedures in collaboration with lung transplant team and other research groups on campus.
Interact and coordinate with off-campus collaborators samples shipments, common on-line meetings, and in-person visits.
Prepare a quarterly report on the performance of the scientists and students, under direct supervision.