Viral Vector Scientist - Gene Therapy

Neion Bio•New York, NY

3d•$120,000 - $150,000•Onsite

About The Position

Neion Bio is harnessing nature’s best molecular factory, the chicken egg, to make medicine affordable, accessible, and resilient. Using cutting edge genetic engineering and stem cell technology, we create “living bioreactors”, which act as fully autonomous 3D biomolecular printers, operating on existing industrial-scale infrastructure. Our platform can rapidly and scalably produce almost any protein, provide national resilience to drug shortages and de-risk complex supply chains, and ultimately take advantage of novel biological environments to solve unmet medical needs. We are on a mission to reimagine the way in which complex biological molecules are produced to transform human health. At Neion Bio you will play a pivotal role in an early-stage, fast-growing company, working alongside leading scientists and entrepreneurs. You will have the opportunity to participate in the enormous potential upside while having a huge impact on the world.

Requirements

PhD (or BA with 7+ years of experience/MS with 5+ years of experience) in biological sciences, bioengineering, molecular biology, genetics, or any science related field, or equivalent experience
Experience with cloning large libraries or large viral genomes
Experience with transfection and transduction of difficult target cells/tissues
Experience producing, purifying, characterizing, validating and testing of a range of viral vectors including adenovirus, lentivirus, AAV
Deep understanding of the cellular biology, receptors for viral entry and trafficking, mechanisms involved in viral transduction, and immunology
Experience with ultracentrifugation and with resin and column based viral purification methods
Experience in genome engineering, cloning, DNA amplification, PCR, NGS, transfections, and other molecular biology skills
Strong aseptic techniques and GLP procedures
Exceptional organizational skills with the ability to effectively manage time and priorities
Strong written and oral communication skills, as well as creative problem-solving skills
Curious, eager, and hardworking

Nice To Haves

Experience working with Avian viruses or AAVs or adapting viral vector strategies for use in avian systems
Extensive experience with and knowledge of adenoviral vectors
Experience with viral capsid engineering by rational design or directed evolution
Experience with viral capsid library engineering

Responsibilities

Perform cloning of large viral genomes, with a focus on capsid modification by either rational design or directed evolution
Perform transfections on primary cells, immortalized cells, and animal tissues
Design, manufacture, purify, characterize, validate and test a range of viral vectors, at small and large scale
Design experiments, generate and analyze datasets allowing to adapt vector strategies to use in avian systems
Assess efficiency of workflows through cellular assays and NGS sequencing approaches
Extract DNA, RNA, protein from animal tissue, design and run PCR, qPCR, RT-PCR, ELISA assays to quantify viral transgene and transcription, protein
Conduct experiments and work on research problems
Maintain excellent electronic laboratory records
Work on project milestones
Attend lab meetings, prepare reports and presentations on the progress of projects