Study Support Technician II – Technical (Aquatics)

Howard Hughes Medical Institute•Ashburn, VA

7d•Onsite

About The Position

The Howard Hughes Medical Institute’s Janelia Research Campus is a pioneering research center in Ashburn, Virginia, where scientists pursue fundamental questions in the life sciences. Our integrated teams of biologists, computational scientists, and tool-builders innovate research practices and technologies to solve biology’s deepest mysteries. HHMI launched Janelia in 2006, establishing an intellectually enriching environment for scientists to do creative, collaborative, hands-on work. We share our methods, results, and tools with the scientific community. Janelia Research Campus is an HHMI research institute built to tackle scientific problems beyond what any single lab or company could sustain. Janelia's next decade centers on two big bets: mapping how a vertebrate brain produces complex behavior, and building “AI-in-the-Loop Discovery,” where scientists and AI work together to analyze data, design experiments, and drive discovery at a new scale. Larval zebrafish and Danionella are key to the first bet. Zebrafish are where Janelia is building the whole-brain imaging pipelines that a viable Danionella platform will depend on, and Danionella — transparent throughout its life — offers a path toward imaging the whole brain of an adult vertebrate during natural, complex behavior, something never before achieved. As this work scales up, Janelia's AAALAC-accredited Vivarium is growing its Aquatics program. This position provides specialized technical services within Janelia's Aquatics program of the Vivarium. These services focus on creating and maintaining genetically modified zebrafish and Danionella colonies in support of Janelia’s research program. Working under minimal supervision, provides expertise with hands-on technical procedures to enable and streamline research.

Requirements

Associate's or Bachelor's degree in life sciences or a related field, or an equivalent combination of education and experience.
Two years of experience with Aquatic husbandry or laboratory animal care, preferably in a research setting.
Demonstrated ability to perform moderate technical procedures, such as proficient microscope use, embryo injection, fin clipping, embryo screening, and cross setup/breakdown.
Comfort using database systems for animal recordkeeping.
Enthusiastic and self-motivated individual, with the ability to work independently with minimal supervision and learn tasks quickly.
Excellent skills in Microsoft Office.
Demonstrated ability to work in a collaborative, rapidly changing team environment to accomplish mutual goals.
Excellent interpersonal and communication skills to deal effectively with research scientists, laboratory technical staff, administrative staff, management, outside contractors, and vendors.
Remaining in a normal seated or standing position for extended periods of time; reaching and grasping by extending hand(s) or arm(s); dexterity to manipulate objects with fingers, for example using a keyboard; communication skills using the spoken word; ability to see and hear within normal parameters; ability to move about workspace.
The position requires mobility, including the ability to move materials weighing up to several pounds (such as a laptop computer or tablet).

Nice To Haves

experience with transgenic colony management is a plus.
Experience with reproductive techniques such as microinjection, IVF, or related molecular biology methods is a plus.

Responsibilities

Perform technical procedures to maintain the zebrafish and danionella colonies, including embryo care, setting up and breaking down crosses, and breeding coordination through establishment of a stable transgenic line.
Perform reproductive techniques, including embryo microinjections, IVF, recovery of cryopreserved sperm, and female ova expression.
Conduct whole animal screening, including fluorescent and/or phenotype screening, dye screening, embryo screening, fin clipping (larval, juvenile, and adult), and sperm collection for PCR.
Track each stage of the microinjection process and provide clear handoffs to Aquatics staff and researchers as embryos progress through early development.
Maintain transgenic colony records on related data systems, including crossing events, genotyping results, screening observations, injection outcomes, AI-supported screening data, and live-feed culture records that support colony nutrition.
Communicate with researchers on breeding colony status, microinjection progress, and other updates as needed.