Staff Research Associate - Pediatric Neonatology

University of California, San Francisco • San Francisco, CA

7d • Onsite

About The Position

The Petersen Lab at UCSF and Gladstone Institutes is seeking a skilled Staff Research Associate (SRA) to join our group. We are studying the underlying mechanisms of neurological disease with a goal of developing new treatments to aid repair in the diseased or injured brain. The candidate will be a critical member of our team who will assist projects focused on animal and cell models of neurological disease. This position involves: Managing animal colonies including breeding, genotyping, and complex crosses to obtain cell-specific knock out mice and fluorescent reporter mice Isolating and culturing cells to study neurological disease processes Preparing brain and spinal cord samples for histology including animal perfusion, tissue dissection, cryosectioning, immunohistochemistry, microscopy, and image analysis using ImageJ Isolating RNA and protein from biological samples for real-time PCR and Western blot Preparation of data and methodology for reports and publications with the possibility for co-authorship when appropriate. The ideal candidate would be someone who is passionate about working with cutting edge technologies to study neurological disease models. Technical accuracy, reliability, and a willingness to learn are of the utmost importance.

Requirements

• One year of recent, directly related work experience following degree completion, utilizing the techniques or methods required by the position; or equivalent combination of education and experience
• BA/BS in a biology or chemistry-related science
• Knowledge of scientific equipment (centrifuge, pipettes, ultra-low freezers)
• Ability to perform repetitive tasks routinely (genotyping, histology protocols, etc)
• Passion to learn new techniques and flexibility to adapt to changing needs
• Ability to multi-task and prioritize
• Excellent teamwork skills and the ability to communicate effectively and collaboratively with students, postdoctoral fellows, staff, and faculty
• Meticulous record keeping abilities
• Strong computer skills including the use of Microsoft Word and Excel

Nice To Haves

• Experience with animal procedures and histology
• Experience with microscopy and image analysis
• Experience with cell culture
• Actively seeks opportunities to increase skills and expertise

Responsibilities

• Manage animal colonies including breeding, genotyping, and complex crosses to obtain cell-specific knock out mice and fluorescent reporter mice.
• Isolate and culture cells to study neurological disease processes.
• Prepare brain and spinal cord samples for histology including animal perfusion, tissue dissection, cryosectioning, immunohistochemistry, and microscopy.
• Quantify microscopy imaging data using ImageJ. Perform calculations, compile and summarize data, keep meticulous records of every experiment.
• Isolate RNA and protein from biological samples for real-time PCR and Western blot.
• Maintain stocks, reagents, laboratory equipment, perform other duties as assigned.

What This Job Offers

• Job Type: Full-time
• Career Level: Entry Level