Single Cell Genomics / Molecular Biologist

University of British Columbia•Vancouver, BC

27d

About The Position

We are developing new single-cell genome and transcriptome methods for tracking clonal trajectories at the single-cell level in patient tissues and to gain a better understanding of the tumour microenvironment. Our researchers use the latest single-cell genomics technologies to rapidly identify genes that are involved in the development of cancer. This position will be working on the Single-Cell Genomics Platform on research projects currently ongoing. The successful candidate will be providing technical guidance, expertise, technology development and support to both internal/external researchers using the single-cell genomics platform, located at two separate research facilities in Vancouver, B.C. We are seeking a highly motivated and highly skilled individual with expertise or experience in single-cell genomics, liquid handling robotics, and/or next-generation sequencing with a strong background in molecular biology. The successful applicant will work full-time under the direction of Dr. Adi Steif, collaborating Principal Investigators including Drs. Samuel Aparicio and Pamela Hoodless, and their designates. Individuals with experience using liquid handling robotics and Next-Generations Sequencing techniques are considered an asset. This position will provide the candidate with a high level of exposure to single-cell genomics and translational research, as well as providing opportunities to work collaboratively with industry partners and other researchers globally; the position start date is flexible.

Requirements

Completion of a university degree in a relevant discipline or technical program and a minimum four years of related experience or an equivalent combination of education and experience.
Some positions may require a graduate degree.
Willingness to respect diverse perspectives, including perspectives in conflict with one’s own.
Demonstrates a commitment to enhancing one’s own awareness, knowledge, and skills related to equity, diversity, and inclusion.

Nice To Haves

An MSc in Biological Sciences (Molecular Biology or other relevant fields) with work experience in single-cell genomics biology, next-generation sequencing or molecular biology is preferred.
Working knowledge and understanding of molecular biology and biochemistry techniques such as next-generation sequencing, PCR, DNA/RNA purification and molecular cloning techniques are essential; prior work experience in next-generation sequencing and library construction is considered a great asset.
Experience with cell culture and aseptic techniques.
Working hands-on experience in tissue handling and tissue dissociation methods would be an asset.
Ability to perform and troubleshoot a wide variety of molecular and cellular biology techniques including the following: RNA/DNA isolation, quantitative PCR, transfection, flow cytometry, immunohistochemistry, ELISA, protein isolation, and western blotting.
Preference will be given to applicants with liquid handling robotics, single-cell genomics, next-generation sequencing, and 10x Genomics experience.
Demonstrated understanding of experimental design and assay optimization.
Strong interpersonal skills, communication skills (oral and written) and the ability to interact positively and productively with other team members are essential.
Excellent documentation and organizational skills.
Able to multi-task, prioritize and handle multi-project assignments concurrently.
The successful candidate must be well-organized, conscientious, understand the importance of detail, and be able to multi-task and prioritize duties effectively.

Responsibilities

Plans and performs experiments using established complex procedures and techniques to investigate single-cell genomics, including the use of liquid handling robotics and 10x Genomics platforms; determines appropriate techniques and modifies or adapts existing methods as needed.
Collaborates with PIs and team members to support the development of project pipelines and technical experiments; incorporates feedback from bioinformatics team members on sequencing data quality to iteratively optimize the library preparation protocol.
Visualize and analyze cells using microscopy and flow cytometry.
Generates, prepares, and submits next-generation sequencing libraries; designs and modifies methods, executes existing protocols, and determines appropriate steps to iteratively optimize experiments.
Using a LIMS system to track and record sample data.
Provide technical expertise and support in molecular biology, protein biochemistry, tissue culture and virology.
Develop and implement standard operating protocols (SOPs).
Maintains laboratory records and experimental data.
Assist in general lab maintenance such as reagent preparation, receiving and inventory of lab supplies.
Conducts complex data analysis and interpretation of data resulting from high throughput experiments.
Presents data at scientific meetings, writes reports and contributes to writing ethics and grant applications.
Carries out any other related duties as assigned or required in keeping with the qualifications and requirements of this technician level.