Senior Research Associate / Scientist, Proteomics Sample Prep

Glyphic Biotechnologies • Berkeley, CA

1d • Onsite

About The Position

About Glyphic: At Glyphic Biotechnologies, we plan to create the protein revolution for which scientists and researchers have been waiting. We are developing a massively parallel, single-molecule proteome sequencing platform that will transform life science discovery and usher in a new era of insights into human biology and disease. We have raised >$80M from venture partners and non-dilutive grant funding. What we are looking for in you We're seeking a hands-on, detail-oriented Senior Research Associate / Scientist I to join our proteomics team and help scale our sample processing capacity. Sample volume is growing quickly, and we need someone who can take on the day-to-day execution of tissue and cell sample preparation and mass spec operation. Reporting to our principal proteomics scientist, you'll spend most of your time at the bench. You'll follow established SOPs closely, prepare and run high volumes of samples, and help keep our Astral mass spec calibrated, well-maintained, and running smoothly. This is a wonderful opportunity to gain hands-on training on the Orbitrap Astral Zoom + Vanquish Neo platform from an experienced mass spec scientist, and growing ownership of the workflow as our automated systems come fully online. This is a full-time, exempt, in-person position with work conducted at our Berkeley, CA location.

Requirements

• Industry or Academic experience relevant to the role and a degree in Proteomics, Chemical Biology, Biochemistry, Analytical Chemistry, or a related field including: BS/MS with 2+/0+ years (Senior Research Associate) BS/MS with 4+/2+ years (Scientist I)
• Hands-on experience with LC–MS/MS–based proteomics
• Experience processing precious, complex clinical samples to include tissue dissection and cell pellet handling
• Demonstrated experience in sample preparation, protein extraction, and enrichment strategies (chemical biology/enrichment strategies preferred)
• Familiarity with data independent acquisition (DIA)
• Excellent problem-solving skills and attention to detail
• Excellent written and verbal communication skills, with the ability to work seamlessly with colleagues from diverse scientific and technical backgrounds
• Ability to thrive in a fast-paced environment with evolving priorities
• Occasional weekend availability required (~1-2 hours)

Nice To Haves

• Experience with Thermo Orbitrap mass specs and Vanquish Neo LCs
• Experience with cell surface proteomics or chemical labeling approaches
• Familiarity with immunopeptidomics workflows (MHC-I/MHC-II)
• Experience with performing LC-MS/MS on low-input samples (single-cell, spatial proteomics, immunopeptidomics)
• Familiarity with high-throughput proteomics workflows, automation and sample multiplexing
• Understanding of protein chemistry, post-translational modifications, and quantitative proteomics
• Basic knowledge of bioinformatics tools or scripting (e.g., R, Python)

Responsibilities

• Prepare tissue and cell samples for mass spectrometry–based proteomics, including precise tissue dissection and cell pellet processing
• Execute established protein and peptide enrichment strategies, including cell-surface labeling and immunopeptidomics workflows
• Follow validated protocols for protein extraction and purification across a range of sample types
• Utilize automation to achieve a sample throughput at 96x plate format scale.
• Learn Orbitrap Astral Zoom operation and troubleshooting
• Operate and maintain the Orbitrap Astral Zoom, including routine instrument calibration
• Follow established SOPs closely to ensure consistent, reliable instrument performance
• Work closely with the proteomics and cross-functional teams to keep sample processing on schedule
• Document methods and maintain SOPs to support consistent, reproducible results
• Communicate proactively, surfacing issues early rather than letting them surface downstream

Benefits

• Employee Stock Option Plan
• 100% Health Plan Coverage for Employees & Dependents (Medical, Dental, & Vision)
• Employer Retirement Contributions to 401(k)
• Generous Paid Time Off
• Paid Maternity and Paternity Leave
• Health & Wellbeing Program
• Office Snacks and Beverages
• Regular Team Bonding Activities

What This Job Offers

• Job Type: Full-time
• Career Level: Senior
• Education Level: Associate degree