Senior Research Associate, In Vivo Pharmacology

Unither Pharmaceuticals•Cambridge, MA

5d•$79,000 - $105,000•Onsite

About The Position

The Senior Research Associate, In Vivo Pharmacology will play a key role in advancing Thymmune's thymic rejuvenation programs by conducting in vivo studies and performing comprehensive analytical assessments to evaluate the efficacy and safety of iPSC-derived thymic cell therapies. This individual will establish and execute animal models to support proof-of-concept and pharmacology studies, driving the generation of high-quality preclinical data to enable nonclinical development.

Requirements

5+ years of experience conducting in vivo pharmacology studies, including study execution, animal handling, dosing, sample collection, and tissue processing with a Bachelor's Degree in immunology, regenerative medicine, or a related life sciences discipline or 3+ years of experience conducting in vivo pharmacology studies, including study execution, animal handling, dosing, sample collection, and tissue processing with a Master's Degree n immunology, regenerative medicine, or a related life sciences discipline
In Vivo Skills: Demonstrated proficiency in rodent handling, tissue collection/dissection, and multiple routes of administration, including intravenous (IV), intraperitoneal (IP), and subcutaneous (SC) injections., and tissue dissection is required
Analytical Skills: Strong experience with Flow Cytometry, qPCR, ELISA/MSD techniques. Demonstrated ability to analyze data, apply appropriate statistical methods, and independently troubleshoot experimental variability.
Communication and collaboration; Excellent oral and written communication skills. Must be able to structure tasks, set priorities, and work effectively in a collaborative, team-oriented environment.

Nice To Haves

1+ year of experience in the biotech and/or biopharma industry
In Vitro Skills: Hands-on experience with the isolation, processing, and culture of primary human cells or tissues is preferred.
Scientific knowledge: Fundamental knowledge of thymus biology and immunology or demonstrated ability to rapidly acquire expertise in these areas, is preferred.

Responsibilities

Perform standard dosing procedures (SC, IM, IV, IP) for cell administration, blood sampling techniques (submandibular bleeds and tail nick) for immune cell profiling, as well as tissue collection (e.g., lymph tissue, muscle, and major organs) via necropsy
Perform cell isolation from primary tissues and conduct downstream biological assays to evaluate cell quality and function, including Flow Cytometry (FACS), RT-qPCR/ ddPCR, MSD/ELISA, Immunofluorescence imaging
Maintain high standards of data integrity and ensure accurate, reproducible, and well-documented data to support reliable analysis and decision-making. Analyze complex datasets using statistical software (e.g., GraphPad Prism) and effectively present findings to the scientific team.
Document in vitro and in vivo studies using electronic notebook (e.g., Benchling) to maintain detailed records of study design, experimental procedure, analytical data, and results
Lead study planning and organization, write study reports and participate in the strategic planning of long-term studies
Perform specialized tissue processing and in vivo imaging analysis, including immunohistochemistry (IHC) and IVIS-based reporter gene imaging
Develop and execute immunological assays (e.g., cell activation, cytotoxicity, mixed lymphocyte reaction) to assess the allo-reactivity and tolerance of immune cells
Conduct and/or assist with small rodent surgical procedures (e.g., tissue transplantation, cell implantation), experience in surgical procedures is a key asset
Maintain cell culture of cell line or primary immune cells using aseptic techniques
Ensure strict adherence to all Institutional Animal Care and Use Committee (IACUC) guidelines and standard operating procedures (SOPs) for animal welfare
Collaborate cross-functionally to support timely execution of in vivo studies to characterize and select candidate cell therapy product
All other duties as required