Senior Molecular Biology Associate I PC 1406

Miltenyi Biotec•Gaithersburg, MD

1d•Onsite

About The Position

The role will support the development of new plasmid DNA purification processes for preclinical and GMP manufacturing. The role will also involve supporting ongoing construct assembly and plasmid production. This position requires a hands-on approach and relies on strong expertise in bacterial systems, plasmid purification, and molecular biology techniques to help optimize processes, design experiments, analyze data, and prepare technical documentation within a collaborative, cross-functional environment. Miltenyi Biotec is passionate about making a real impact, playing a pivotal role in the design, development, manufacture, and integration of products for sample preparation, cell separation, cell analysis, imaging, and cell culture needs. These developments have led to cutting-edge cell and gene therapies – transformative methods that mobilize the body’s own cells and genetic blueprint to tackle diseases such as cancer and autoimmune conditions. Today, their more than 18,000 solutions play a vital role in paving the way for the medicine of tomorrow.

Requirements

Bachelor’s degree in molecular biology, Biochemistry, Biotechnology, or a related scientific discipline with +5 years of hands-on experience.
Hands-on experience with plasmid DNA purification.
Experience with molecular biology techniques, including cloning, restriction enzyme digestion, sequencing analysis, qPCR, and ddPCR.
Experience designing and assembling constructs using molecular biology and sequence analysis software.
Experience supporting process development or optimization activities in a PD/R&D environment.
Familiarity with GMP concepts and regulated documentation practices.
Strong working knowledge of bacterial culture protocols, including aseptic technique and safe handling of biological materials.
Strong attention to detail, organization, and data integrity.
Clear written and verbal communication skills and ability to work in a team-based environment.

Responsibilities

Perform molecular biology assays, including qPCR, ddPCR, restriction enzyme digest, and other related analytical techniques.
Execute on plasmid DNA production activity according to defined Standard Operating Procedures (SOPs) and Batch Production Records (BPRs).
Develop, optimize, and troubleshoot plasmid purification processes with a focus on scalability and GMP readiness.
Author and review protocols, reports, and standard operating procedures (SOPs).
Perform molecular cloning and construct assembly, including restriction digests, ligations, transformations, and clone screening.
Design DNA constructs using molecular biology software and analyze sequencing data to confirm construct identity and integrity.
Maintain accurate experimental records in accordance with cGMPs.
Coordinate preventive and emergency maintenance, while demonstrating proper equipment use.
Assist the Department in identifying and executing ongoing improvement initiatives to meet operational objectives.
Collaborate effectively within the molecular biology team and across cross-functional teams.