Scientist in Protein Technologies Center - Cell Engineering / Molecular Biology
About The Position
The Protein Technologies Center (PTC), within the Department of Structural Biology, is seeking a scientist skilled in saturation-mutagenesis-reinforced functional assays, site-specific integrase-based stable expression platforms, and genome-editing technologies. The scientist will systematically tag endogenous proteins with fluorescent and affinity tags, enabling structural, functional, and drug-discovery studies, and supporting investigations of protein interactions and localization. The PTC is a multidisciplinary team spanning protein engineering, cloning, expression, purification, biophysical analysis, and structure determination. In this bench-based role, the scientist will edit cell lines, confirm successful editing, and verify genomic stability throughout the clonal line-generation process. The role provides hands-on support to structural cell biology projects across the department, both strategically and tactically.
Requirements
- Bachelor's degree in a relevant scientific area is required.
- Eight years of relevant work experience with a bachelor's degree, six years with a master's degree, or three years with a PhD.
- Proven track record in molecular cloning, including methods such as In-Fusion, LIC, PIPE, Gateway, FX, overlap extension PCR, Gibson assembly, BioBrick, or recombineering.
- Experience handling large and/or complex DNA constructs, including combinatorial libraries.
- Experience with site-directed and/or saturation mutagenesis.
Nice To Haves
- PhD in molecular biology, synthetic biology, or a related discipline, with at least five years of postdoctoral experience.
Responsibilities
- Engineer cell lines using CRISPR gene-editing technology to generate gene knockouts and knock-ins, including SNVs and gene tags, and confirm the edits.
- Generate stable cell lines using non-viral and viral-based delivery systems.
- Implement advanced technologies to develop stable target-overexpressed cell lines, pools, clones, and NGS library preparation.
- Systematically tag endogenous proteins with fluorescent and affinity tags to enable structural, functional, and drug-discovery studies and investigations of protein interactions and localization.
- Verify genomic stability throughout the clonal line-generation process.
Benefits
- Medical and prescription drug plans
- Dental plan
- Vision plan
- Financial security benefits
- Retirement plan
- Employee wellness program
- Time off programs
- Educational benefits
- Family-friendly benefits
- Benefits unique to St. Jude
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What This Job Offers
Job Type
Full-time
Career Level
Senior
