Scientist

Axle•Rockville, MD

3d•$90,000 - $95,000•Onsite

About The Position

Axle is a bioscience and information technology company that offers advancements in translational research, biomedical informatics, and data science applications to research centers and healthcare organizations nationally and abroad. With experts in biomedical science, software engineering, and program management, we focus on developing and applying research tools and techniques to empower decision-making and accelerate research discoveries. We work with some of the top research organizations and facilities in the country including multiple institutes at the National Institutes of Health (NIH). Axle is seeking a Scientist to join our vibrant team at the National Institutes of Health (NIH) supporting the National Institute of Child Health and Human Development (NICHD) located in Bethesda, MD.

Requirements

Master's degree in Biology, Life Sciences, or a related discipline
Extensive experience as lead investigator on research projects
Experience with protein assays (e.g., Western Blotting)
Experience with transfection experiments
Experience with DNA cloning
Experience with phenotyping of mutant mouse strains
Experience with protein function assays
Experience with mouse colony maintenance
Experience with cell culture
Experience with fetal thymus organ culture
Experience with analyzing and summarizing data
Proficiency in PowerPoint, Outlook, Excel, and Word

Nice To Haves

Field of Study: Genetics
Experience with multicolor flow cytometry
Experience with phosphatase assay
Experience with GTPase assay
Experience with animal tracking and genotyping by PCR
Experience mentoring post-baccalaureate fellows
Experience assisting post-doctoral fellows
Experience maintaining laboratory notebooks and databases
Experience participating in laboratory meetings and discussions
Experience performing literature searches
Experience assessing project direction
Experience identifying new projects and research directions
Experience maintaining laboratory equipment and training others
Experience managing scientific equipment and resources
Experience attending international scientific meetings
Experience presenting findings in oral presentations or as posters
Experience reviewing scientific papers
Experience writing invited review articles and technical experimental methods articles

Responsibilities

Support the planning and execution of research experiments within the laboratory's overall research program.
Perform protein assays, transfection experiments, DNA cloning, and phenotyping of mutant mouse strains, along with protein function assays and mouse colony maintenance.
Support laboratory staff on research projects and techniques, including mentoring post-baccalaureate fellows and assisting post-doctoral fellows with experiments in protein biochemistry.
Evaluate, analyze, and interpret research data, communicating results and methods in reports, presentations, and manuscripts.
Perform protein assays by Western Blotting to detect protein expression and protein associations in T cells from mice.
Perform transfection experiments with plasmids and retroviruses into cell lines or primary mouse cells.
Perform DNA cloning and generate constructs for gene expression and gene targeting in ES cells.
Perform phenotyping of mutant mouse strains by multicolor flow cytometry.
Perform protein function (signaling) assays on thymocytes and T cells.
Perform and optimize less common assays, such as phosphatase assay and GTPase assay.
Perform cell culture and fetal thymus organ culture.
Maintain a mouse colony for experiments.
Participate in animal tracking and genotyping by PCR.
Prepare media, buffers, and chemical reagents as needed.
Assist with mentoring and teaching of post-baccalaureate fellows.
Assist post-doctoral fellows in their independent projects through consultation and by performing selected key experiments within the contractor's area of expertise (protein biochemistry).
Maintain and update laboratory notebooks and databases; collect, analyze, and summarize data for reports.
Participate in laboratory meetings and discussions; review literature for assay techniques and topics of interest.
In consultation with staff, assess project direction, perform literature searches, and assist with identifying new projects and research directions to pursue.
Perform key pilot experiments to test new hypotheses forming the foundation of new research projects.
Maintain laboratory equipment and train others in its use.
Maintain laboratory supplies and equipment.
Assist in maintaining adequate space and facilities for ongoing and projected research studies.
Manage scientific equipment and resources (e.g., reagents, frozen cell stocks, animal samples), including acquisition, documentation, and storage.
Attend international scientific meetings and present findings in oral presentations or as posters, as allowed per STOPS Contract Travel SOP.
Assist staff in reviewing scientific papers and writing invited review articles and technical experimental methods articles in specific areas of expertise.
Analyze and summarize data for reporting results; consult with staff regarding techniques and results.