(Senior) Scientist, Translational Biology

Flagship Pioneering, Inc.•Cambridge, MA

9h•$126,000 - $165,000•Onsite

About The Position

We are seeking a highly motivated and experienced Scientist to join our research team. The ideal candidate will have deep expertise in flow cytometry, primary cell isolation and sorting, microscopy and imaging, and gene-silencing techniques (siRNA), with in vivo/in vitro mouse experience. This role will contribute to the design and execution of preclinical studies to advance therapeutic programs.

Requirements

Ph.D. in Molecular Biology, Cell Biology, or a related field with 3–6+ years of relevant experience (industry preferred), or M.S. with significant experience
Strong expertise in flow cytometry, including cell sorting (FACS), panel design, troubleshooting, and data analysis (e.g., FlowJo or similar tools)
Hands-on experience with primary cell isolation techniques from mouse tissue, including tissue dissociation (enzymatic and mechanical), magnetic bead-based separation (MACS), and isolation of rare or fragile cell populations
Hands-on experience with microscopy techniques (e.g., immunofluorescence, confocal microscopy) and associated image analysis software (e.g., ImageJ/Fiji, Imaris, or similar)
Demonstrated experience with siRNA design, delivery, and functional validation
Strong data analysis and problem-solving skills
Excellent communication and presentation skills
Ability to work independently and collaboratively in a fast-paced environment

Nice To Haves

Experience with mouse models, including in vivo study design, dosing, and tissue collection
Experience in translational or preclinical research environments
Experience with cold-active or optimized dissociation protocols for isolating sensitive/rare cell types (e.g., podocytes, neurons, microglia) while minimizing dissociation-induced expression artifacts
Experience with advanced imaging modalities (e.g., intravital microscopy, super-resolution, high-content imaging)
Familiarity with additional molecular techniques (qPCR, Western blot, ELISA, RNA-seq, gene silencing, protein over-expression)
Experience working in a biotech or pharmaceutical setting

Responsibilities

Design, execute, and analyze complex in vitro / in vivo experiments to support research programs
Lead and perform multi-parameter flow cytometry assays, including panel design, optimization, and data interpretation
Perform and optimize primary cell isolation from tissues, including enzymatic and mechanical tissue dissociation, magnetic-activated cell sorting (MACS), and fluorescence-activated cell sorting (FACS), with attention to preserving cell viability, yield, native gene-expression signatures, and potential for further culture of the cells
Perform and interpret microscopy-based assays, including high-content microscopy, immunofluorescence, confocal, and/or intravital imaging, to characterize cell and tissue phenotypes and validate target engagement
Support mouse studies as needed, including disease models, dosing, tissue collection, and phenotypic analysis
Develop and apply siRNA-based approaches for target validation and mechanistic studies
Analyze and interpret data, generate reports, and present findings to cross-functional teams
Collaborate with internal and external stakeholders to advance project goals
Mentor junior scientists and contribute to a collaborative team environment
Maintain accurate experimental records and ensure compliance with institutional and regulatory guidelines