Scientist, Tissue Engineering

Unither Pharmaceuticals•Manchester, NH

12d•Onsite

About The Position

United Therapeutics (Nasdaq: UTHR) is a publicly-traded public benefit corporation dedicated to developing novel pharmaceutical therapies and technologies for rare, deadly diseases and to address the shortage of transplantable organs. The company has six FDA-approved therapies and is developing treatments for pulmonary arterial hypertension (PAH), pulmonary fibrosis (PF), and other conditions. A significant focus is on creating manufactured organs to eliminate transplant waiting lists. The Scientist, Tissue Engineering will contribute to advancing tissue engineering strategies to create living 3D printed lung scaffolds within the Organ Manufacturing Group (OMG). This role involves designing and executing experiments to generate multicellular lung scaffolds and developing analytical methods to assess their functionality.

Requirements

Doctor of Philosophy (PhD) in Biology, Biomedical Sciences, Tissue engineering, Bioengineering (or equivalent)
3+ year(s) prior research experience in a field related to pulmonary biology, endothelial biology, iPSC-derived lineages, tissue engineering, and/or culturing cells in novel contexts. Relevant experiences during graduate school will be considered.
2+ years of industry and/or postdoctoral training
Strong technical experience including imaging techniques (e.g. basic microscopy, confocal imaging, image analysis) and biological analytical assays (e.g. immunocytochemistry, gene expression analysis, ELISAs, flow cytometry, viability assays, etc)
Track record of success (publications, products, patents, grants, or fellowships) with minimum one first-author publication in a related or comparable field.
Experience in endothelial cell biology or vascular tissue engineering with supporting experience or knowledge of pulmonary biology, epithelial cells, and/or stem cell-derived lineages
Understanding of tissue engineering concepts and current field
In-depth knowledge of endothelial cells and biology
Experience with cell-cell interactions, ECM production, or co-culture techniques
Ability to make appropriate recommendations for long term project plans based on scientific data
Familiarity with cell-blood interactions, thrombogenic and inflammatory responses
Experience with imaging and microscopy
Experience with perfusate analytics, secretome characterization, cellular metabolism, gene expression profiling, and cell-cell signaling applicable to complex tissue culture systems
Experience or interest in large-scale bioreactors or perfusion culture systems

Nice To Haves

2+ years of experience in leading a project team with a track record of success (e.g. product, grants, and/or publications)
Methods to evaluate endothelial cell health in vitro (tube formation angiogenesis assay)
Proficient with microfluidics, bioreactor and/or other dynamic culture techniques

Responsibilities

Design and execute in vitro experiments using adult and iPSC-derived lung cells (endothelial and epithelial) to develop functional tissue on 3D-printed models.
Develop and implement strategies and analytic assays to optimize culture systems, co-culture techniques, and large-scale bioreactors for pulmonary tissue engineering.
Develop and implement analytical assays (immunocytochemistry, biochemical assays, metabolic readings, perfusate analysis, gene expression analysis) to evaluate tissue formation and maturation on 3D printed models.
Record experimental details and findings in electronic notebook entries, write project reports, and internally present research findings.
Coordinate with supervisor for project directions, experimental planning, and to review results and progress.
Contribute to IP, regulatory filings, and journal publications, if necessary.
Maintain high level of communication with internal cross-functional R&D team, as well as external vendors and consultants.
Perform other duties as assigned.