E11 Bio - Scientist/Senior Scientist, Molecular Engineering & Sample Optimization
About The Position
E11 Bio is seeking a mission-aligned experimental scientist with an engineering approach to the full pipeline, from sample preparation through molecular readout tools. This role owns both optimizing the physical preparation of the tissue and engineering the reagent panels across two target types: comprehensive synapse identification and barcode epitopes. Reporting to the Barcoding Lead, you will define what high-quality tissue means quantitatively and will drive the improvement of high quality morphology and protein readout by working closely with molecular biology, imaging, and computational teams to support whole-brain connectomics.
Requirements
- Experimental scientist
- Bench, microscope, and quantitative data analysis skills
- Engineering approach to sample preparation through molecular readout tools
- Experience optimizing physical preparation of tissue
- Experience engineering reagent panels for synapse identification and barcode epitopes
- Ability to define quantitative metrics for high-quality tissue
- Experience working with molecular biology, imaging, and computational teams
- Experience with assay development
- Experience with quantitative metrics of molecular readout (protein retention, antigenicity, labeling quality)
- Experience designing experiments and optimizing SOPs
- Experience developing data-analysis pipelines
- Experience with binder design/discovery, validation, and optimization
- Experience developing multiplexed protein readout strategies
Nice To Haves
- Experience in connectomics
- Experience in neuroscience
- Experience in mammalian brain mapping
- Experience with hydrogels
- Experience with synaptic protein annotation
- Experience with cell type annotation
Responsibilities
- Develop and deploy an assay to systematically evaluate and improve the fixation quality and preservation of brain tissue
- Establish and integrate quantitative metrics of molecular readout e.g. protein retention, antigenicity, and labeling quality across tissue preparation conditions
- Work closely with in vivo research technicians to design experiments, evaluate results, and optimize SOPs
- Drive the development of data-analysis pipelines to routinely generate key performance indicators of sample tissue quality
- Generate an in-house pipeline for binder design/discovery, validation, and optimization of epitope-binder pairs in hydrogels to improve the readout of epitope barcode bits and relevant native synaptic proteins
- Develop novel highly multiplexed protein readout strategies for protein barcodes and molecular annotation of synapses and cell types
Benefits
- Team performance based bonus up to 20% of base salary
- 401k match up to 6% of base salary
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What This Job Offers
Job Type
Full-time
Career Level
Mid Level
Education Level
No Education Listed
