Scientist / Senior Scientist, Assay Development (Sample Prep)

Glyphic Biotechnologies • Berkeley, CA

10d • Onsite

About The Position

Glyphic Biotechnologies is developing a massively parallel, single-molecule proteome sequencing platform to transform life science discovery. We are seeking a technically driven, detail-oriented, and collaborative Scientist to support sample preparation method development. This role involves testing and optimizing Glyphic’s single-molecule protein sequencing platform to achieve industry-leading metrics for robustness, sensitivity, and scalability. A critical component of this work involves developing and integrating specialized sample preparation protocols for diverse biological inputs with stringent downstream requirements for high-purity and high-accuracy proteomic applications. The ideal candidate will independently troubleshoot complex technical challenges, rapidly iterate on experimental designs, and communicate effectively across multidisciplinary teams. A track record of adapting protocols for novel sample types and executing within high-throughput environments will be key to making an immediate impact. This is a full-time, exempt, in-person position located in Berkeley, CA.

Requirements

• Degree in biochemistry, chemical biology, molecular biology, or an equivalent field.
• PhD / MS / BS with 4+ / 6+ / 8+ years of relevant experience (Senior Scientist) OR PhD / MS / BS with 0+ / 2+ / 4+ years of relevant experience (Scientist I)
• Demonstrated expertise in developing and optimizing ultrasensitive proteomic assays from real biological samples, with experience generating quantitative readouts across diverse analytical platforms (e.g., LCMS for discovery proteomics).
• Experience processing real biological samples from various sources (cell lines, blood, tissue, etc.)
• Expertise with developing sample preparation or enrichment methods for low-abundance targets or limited input material.
• Understanding of peptide chemistry and strategies to prevent degradation of post-translational modifications (PTMs), such as methylation and phosphorylation.
• Excellent problem-solving skills and strong attention to detail.
• Excellent written and verbal communication skills, with the ability to collaborate effectively across diverse scientific and technical backgrounds.
• Ability to thrive in a fast-paced environment with evolving priorities.

Nice To Haves

• Deep familiarity with highly multiplexed, high-sensitivity protein quantification platforms (e.g., nELISA, NuLISA, immunoassays, PLE, or PEA).
• Experience with nanopore platforms for sequencing or label-free detection.
• Experience with single-cell or spatial proteomics and transcriptomics.
• Comfort working with R or Python to input experimental results and navigate established data analysis workflows.

Responsibilities

• Design, test, and optimize inputs for ProSE experiments for robustness, sensitivity, and scalability, including improving reaction efficiency and accuracy.
• Lead the integration of novel chemistry developments into sample preparation and assay workflows, driving high-precision proteomic peptide generation with an eye toward reproducibility, automation, and scalability.
• Optimize and integrate C-terminal and side-chain blocking chemistries - developed by the Chemistry team - into automated assay workflows.
• Propose and qualify in-process controls and analytical tools to build a comprehensive understanding of the factors driving sequencing efficiency across peptides with diverse physicochemical properties, including length, hydrophobicity, and charge.
• Apply chemoenzymatic synthesis approaches and characterize individual enzymatic reaction steps to evaluate efficiency, selectivity, and compatibility.
• Introduce and validate real biological sample inputs, enabling sequencing of diverse, relevant material across a range of sample types and complexities.
• Evaluate and process incoming Key Opinion Leader (KOL) samples, ensuring preparation methods accommodate sample-specific constraints and analytical requirements.
• Assess diverse sample types, determine appropriate analytical approaches, and develop extraction and purification protocols for inputs derived from matrices such as cells, tissues and blood, collaborating with chemists to scale and integrate these methods into high-throughput workflows.
• Develop and implement strategies for sample enrichment, depletion, and dynamic range management to optimize ProSE sequencing yield.
• Support the generation and sourcing of standard reference materials using cell culture techniques, including establishing the instrumentation needed to produce and purify proteins and peptides from cell cultures and other biological materials.

Benefits

• Employee Stock Option Plan
• 100% Health Plan Coverage for Employees & Dependents (Medical, Dental, & Vision)
• Employer Retirement Contributions to 401(k)
• Generous Paid Time Off
• Paid Maternity and Paternity Leave
• Health & Wellbeing Program
• Office Snacks and Beverages
• Regular Team Bonding Activities

What This Job Offers

• Job Type: Full-time
• Career Level: Entry Level
• Education Level: High school or GED