Scientist in Protein Technologies Center - Cell Engineering / Molecular Biology

St. Jude Children's Research Hospital•Memphis, TN

12d•Onsite

About The Position

The Protein Technologies Center (PTC), within the Department of Structural Biology, is seeking a scientist skilled in saturation-mutagenesis-reinforced functional assays, site-specific integrase-based stable expression platforms, and genome-editing technologies. The scientist will systematically tag endogenous proteins with fluorescent and affinity tags, enabling structural, functional, and drug-discovery studies, and supporting investigations of protein interactions and localization. St. Jude Children’s Research Hospital provides world-class facilities, state-of-the-art technologies, extraordinary support, and a collaborative, bench-to-bedside environment. The PTC is a multidisciplinary team spanning protein engineering, cloning, expression, purification, biophysical analysis, and structure determination. This is a bench-based role where the scientist will edit cell lines, confirm that editing was successful, and verify that genomic stability is maintained throughout the clonal line-generation process, providing pivotal, hands-on support to structural cell biology projects across the department, both strategically and tactically. A cover letter is required with the application.

Requirements

Bachelor's degree in relevant scientific area is required.
Eight (8) years of relevant work experience is required with a Bachelor's degree.
Six (6) years of relevant work experience is required with a Master's degree.
Three (3) years of relevant work experience is required with a PhD.

Nice To Haves

PhD (with at least 5 years of postdoctoral experience) in molecular biology or synthetic biology, or any related discipline preferred.

Responsibilities

Engineer cell lines using CRISPR gene editing technology for generating gene knockouts and knock-ins (SNVs, gene tags, etc.) and confirm the edits.
Generate stable cell lines using non-viral and viral-based delivery systems.
Implement advanced technologies to develop stable target-overexpressed cell lines, pools, clones, and NGS library preparation.
Proven track record in molecular cloning (In-Fusion or LIC or PIPE or Gateway or FX; overlap extension PCR/Gibson assembly/BioBrick/recombineering), handling large and/or complex DNA constructs, including combinatorial libraries; site-directed and/or saturation mutagenesis