Scientist II, Structural Biology

Revolution Medicines•Redwood City, CA

6d•Onsite

About The Position

Revolution Medicines is a late-stage clinical oncology company developing novel targeted therapies for patients with RAS-addicted cancers. The company’s R&D pipeline comprises RAS(ON) inhibitors designed to suppress diverse oncogenic variants of RAS proteins. The company’s RAS(ON) inhibitors daraxonrasib (RMC-6236), a RAS(ON) multi-selective inhibitor; elironrasib (RMC-6291), a RAS(ON) G12C-selective inhibitor; zoldonrasib (RMC-9805), a RAS(ON) G12D-selective inhibitor; and RMC-5127, a RAS(ON) G12V-selective inhibitor, are currently in clinical development. As a new member of the Revolution Medicines team, you will join other outstanding professionals in a tireless commitment to patients with cancers harboring mutations in the RAS signaling pathway. We are looking for motivated structural biologist to join tour team to support multiple oncology therapeutic programs. The ideal candidate will have previous, hands-on experience in sample preparation, data acquisition and data analysis from both X-ray crystallography and cryo-EM. They will apply their expertise to form compound driven complexes, identify, and optimize crystallization/CryoEM conditions with the aim of determining high resolution structural information to drive small molecule design and mechanistic understanding. We’re seeking a team player with enthusiasm to work in an interdisciplinary setting to drive toward new, cutting edge oncology therapeutics.

Requirements

PhD in structural biology or protein biochemistry or a related scientific discipline with 2-5 years of post-doctoral experience.
Hands-on experience and expertise in cryo-EM sample optimization and grid preparation, data collection, map generation, and structure determination.
Strong track record of solving challenging CryoEM problems such as preferred orientation, small proteins/complexes, modeling into low resolution maps, or iterative rounds of protein optimization.
Hands on experise with modern software packages for data collection, refinement and visualization of structural biology data, and the ability to seamless switch between them based on need (e.g. CryoSPARC, RELION, Phenix, REFMAC, Coot, ChimeraX, Pymol).
Excellent written, verbal communication, interpersonal and organization skills.
Conscientious team-player interested in a fast-paced biotech environment.

Nice To Haves

Hands-on experience and expertise in protein crystallization, crystal optimization and harvesting for data collection.
Experience with biophysical characterization methods, including mass spectrometry, SEC-MALS, DLS, SPR, NMR and thermal shift assays.
Experience with protein-protein complexes including antibodies/Fabs, molecular glues, PROTACs, etc.
Experience working in a multidisciplinary team environment.
Experience in gene construct design, expression and purification of recombinant proteins.

Responsibilities

Plan and execute experiments with the aim of determining small molecule driven multi-protein complex structures by CryoEM and X-ray crystallography.
Perform cryo-EM grid screening, optimization, data collection and refinement to enable high-quality structure determination.
Interact with interdisciplinary teams (including chemists, computational chemists, biologists, project teams, and management) and clearly articulate structural data in a broader drug discovery context.
Maintain excellent records and communication among internal and external colleagues.
Be driven by a desire to be innovative in a high energy, fast paced environment and work collaboratively with a variety of collegues across the organization.