Scientist I (Gene Therapy/ Cell Cloning)
Dawar Consulting•San Diego, CA
•Onsite
About The Position
The Scientist I will be a vital contributor to our non-viral cell and gene therapy delivery platform, primarily focused on R&D for innovative nucleic acid payload technologies. This role involves evaluating novel mRNA and DNA designs using a range of molecular biology techniques, including plasmid cloning, in vitro validation, and the production of in vitro-transcribed mRNA. To verify successful expression, the scientist will utilize assays such as Western blot, qRT-PCR, and luciferase reporter assays.
Requirements
- Bachelor's degree with 5+ years or a Master's degree with 2+ years of experience in molecular biology, pharmaceutical sciences, or a related field.
- Required proficiency in advanced cloning (Gibson assembly, Golden Gate, site-directed mutagenesis) and IVT.
- Hands-on expertise in measuring transgene delivery success via reporter assays and qRT-PCR.
- Desired proficiency in Snapgene, Prism, and MS Office.
Nice To Haves
- Preferred experience in genetic manipulation of cell lines and primary cells (e.g., T cells, monocytes, hepatocytes).
Responsibilities
- Design and clone expression vectors and IVT donor plasmids to support research initiatives.
- Execute DNA and mRNA transfections in mouse and human cell lines.
- Assess nucleic acid expression through molecular methods like qPCR, reporter assays and Western blotting.
- Maintenance of detailed electronic laboratory notebooks (ELN) and preparation of summary slides
Benefits
- Medical
- Dental
- Vision
- Paid Sick leave
- 401K
Stand Out From the Crowd
Upload your resume and get instant feedback on how well it matches this job.
Upload and Match Resume
What This Job Offers
Job Type
Full-time
Career Level
Mid Level
