Research Technologist (Brain Science Institute)

Johns Hopkins UniversityBaltimore, MD
$16 - $26Onsite

About The Position

We are seeking a Research Technologist who will follow protocols and procedures to perform general laboratory duties and techniques. 50% of the time will be spent learning biochemical techniques, including cell culture, enzyme activity assays, RT-PCR, western blot, and immunohistochemistry. The remaining 50% of the time will be spent performing animal models of peripheral nerve crush and aging.

Requirements

  • Bachelor's Degree in Biology, Chemistry or related field.
  • Additional education may substitute for required experience, and additional related experience may substitute for required education beyond a high school diploma/graduation equivalent, to the extent permitted by the JHU equivalency formula.

Responsibilities

  • Culture and maintain RAW macrophage cells and relevant macrophage, neuronal, Schwann cell, and muscle cell models for efficacy and mechanistic studies.
  • Assist with evaluating glutamate carboxypeptidase II (GCPII) as a therapeutic target in peripheral nerve degeneration and the function of Schwann cells in aging.
  • Study efficacy and pharmacokinetic properties of potential treatments using mouse models of peripheral nerve crush and aging.
  • Maintain aged mouse colonies, including breeding, genotyping, and treating animals.
  • Assist postdoctoral fellow in behavioral and electrophysiological assessments of aging mouse models.
  • Assist in performing histological assessments of therapeutic efficacy in peripheral nerve crush and aging mouse models, including tissue collection, sectioning, and immunohistochemical staining.
  • Perform miscellaneous related duties as assigned.
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